BIOCOMPUTATIONAL GENOME-WIDE ANALYSIS OF MICRO RNA GENETIC VARIABILITY IN SOME VERTEBRATES.

BIOKUMPJUTIZOVANA ANALIZA GENOMA KROZ VARIJABILNOSTI MICRO RNK KOD NEKIH KIČMENJAKA. (Serbian)

MicroRNAs (miRNAs) are small endogenously expressed single-stranded RNAs that regulate gene expression post transcriptionally and shape diverse cellular pathways. miRNAs regulate a wide range of biological processes through the recognition of complementary sequences between miRNAs and their target genes. The present investigation aimed at determining in-silico the genetic variability of miRNA genes in some livestock and non-livestock species. Effects of single nucleotide polymorphisms (SNPs) in genes' 3'UTR on target gain/loss of human miRNAs were also explored. A total of twenty four mature miRNA sequences and genomic coordinates in three livestock [chicken (5), pig (1) and cattle (9)] and two non-livestock (human (6) and mouse (3)] species were retrieved from the miRBase 15 release. Computational scanning of polymorphisms in the miRNAs revealed 33 and 20 polymorphic sites in livestock and non-livestock species, respectively. Of this, 7 (chicken), 11 (cattle) and 2 (mouse) were located within the seed region. The de novo computational prediction revealed that SNPs rs1042725 (C/U) and rs1044129 (A/G) in genes' 3'UTR of human miRNAs positively influenced the target site thereby resulting in target gain. However, the effects of SNPs rs56109847 (A/G), rs28927680, rs12720208 (G/A) and rs5186 (A/C) were negative. The evolutionary tree showed that the relationship between miRNA consensus sequences of livestock (pig, chicken and cattle) was closer compared to non-livestock species (mouse and human), which could be implicated in morphological complexity among vertebrates. Although the function of miRNA is only beginning to be understood, future in-silico research evaluating the functional effect of miRNA in gene translation and subsequent biological pathways especially in livestock is of paramount importance; and this should be complemented with hypothesis-driven experimental studies to evaluate the phenotypic effect of identified miRNA genetic polymorphisms in animals. [ABSTRACT FROM AUTHOR]

Mikro RNKs (miRNKs) regulišu veliki broj bioloških procesa preko prepoznavanja komplementarnih sekvenci izmedu miRNKs i njihovog ciljnog gena. Ispitivanja su obuhvatila in - silico genetičke varijabilnosti miRNK kod domaćih i drugih životinjskih vrsta. Vršena su ispitivanja efekta polimorfizma pojedinačnih nukleotida (SNPs) u genima 3' UTR na cilj povećanje/gubitak humanih miRNKs. Kompjutersko skaniranje polimorfizma u mRNKs je potvrdilo 33 polimorfna mesta kod domaćih životinja i 20 kod ostalih animalnih vrsta. Of this, 7 (chicken), 11 (cattle) and 2 (mouse) were located within the seed region. Evoluciono stablo pokazuje da je odnos između miRNKs sekvenci kod domaćih životinja bliži u poređenju sa mišem i čovekom što može da se uključi u morfološku kompleksnost kičmenjaka. Iako je razumevanje funkcije miRNKs na početku buduća in silico istraživanja i vrednovanje funkcionalnog efekta miRNKs u translaciji i procesima posle translacije, posebno kod domaćih životinja su od velikog značaja. [ABSTRACT FROM AUTHOR]

Copyright of Genetika (0534-0012) is the property of Serbian Genetics Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)