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Elevated Interleukin-32 Expression Is Associated with Helicobacter pylori-Related Gastritis.
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- المؤلفون: Peng, Liu-sheng1; Zhuang, Yuan1 ; Li, Wen-hua1; Zhou, Yuan-yuan2; Wang, Ting-ting1; Chen, Na1; Cheng, Ping1; Li, Bo-sheng1; Guo, Hong2; Yang, Shi-ming2; Chen, Wei-san3; Zou, Quan-ming1
- المصدر:
PLoS ONE. Mar2014, Vol. 9 Issue 3, p1-6. 6p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
Background: Interleukin-32 (IL-32) is a recently discovered proinflammatory cytokine involved in inflammatory diseases. We investigated the expression of IL-32 and its regulation mechanism in the inflammatory response of patients with Helicobacter pylori (H. pylori) infection. Design and Methods: IL-32 mRNA and protein expression in gastric tissues was detected by quantitative real-time PCR and immunohistochemistry. The regulation of IL-32 in human gastric epithelia cell line AGS was investigated by different cytokine stimulation and different H. pylori strain infection. Results: Gastric IL-32 mRNA and protein expression were elevated in patients with H. pylori infection and positively correlated with gastritis. In H. pylori-infected patients, the mRNA level of IL-32 was also correlated with that of proinflammatory cytokines IL-1β and TNF-α. In vitro IL-1β and TNF-α could upregulate IL-32 mRNA and protein level in AGS cells, which was dependent on NF-κB signal pathway. The regulation of IL-32 expression in response to H. pylori-infection could be weakened by using neutralizing antibodies to block IL-1β and TNF-α. Moreover, H. pylori-infected AGS cells also induced IL-32 mRNA and protein expression, which was dependent on CagA. Conclusions: IL-32 level is elevated in patients with H. pylori infection and its expression is regulated by proinflammatory stimuli, suggesting that IL-32 may play a role in the pathogenesis of H. pylori-related gastritis. [ABSTRACT FROM AUTHOR]
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