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Sizing up large protein complexes by electrospray ionisation-based electrophoretic mobility and native mass spectrometry: morphology selective binding of Fabs to hepatitis B virus capsids.
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- المؤلفون: Bereszczak, Jessica1; Havlik, Marlene2; Weiss, Victor2; Marchetti-Deschmann, Martina2; Duijn, Esther3; Watts, Norman4; Wingfield, Paul4; Allmaier, Guenter2; Steven, Alasdair5; Heck, Albert1
- المصدر:
Analytical & Bioanalytical Chemistry. Feb2014, Vol. 406 Issue 5, p1437-1446. 10p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
The capsid of hepatitis B virus (HBV) is a major viral antigen and important diagnostic indicator. HBV capsids have prominent protrusions ('spikes') on their surface and are unique in having either T = 3 or T = 4 icosahedral symmetry. Mouse monoclonal and also human polyclonal antibodies bind either near the spike apices (historically the 'α-determinant') or in the 'floor' regions between them (the 'β-determinant'). Native mass spectrometry (MS) and gas-phase electrophoretic mobility molecular analysis (GEMMA) were used to monitor the titration of HBV capsids with the antigen-binding domain (Fab) of mAb 3120, which has long defined the β-determinant. Both methods readily distinguished Fab binding to the two capsid morphologies and could provide accurate masses and dimensions for these large immune complexes, which range up to ~8 MDa. As such, native MS and GEMMA provide valuable alternatives to a more time-consuming cryo-electron microscopy analysis for preliminary characterisation of virus-antibody complexes. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]
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