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Protein Synthesis Dependence of Growth Cone Collapse Induced by Different Nogo-A-Domains.
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- المؤلفون: Manns, Richard1; Schmandke, Andre2; Schmandke, Antonio2; Jareonsettasin, Prem1 ; Cook, Geoffrey1; Schwab, Martin E.2; Holt, Christine1; Keynes, Roger1
- المصدر:
PLoS ONE. Jan2014, Vol. 9 Issue 1, p1-7. 7p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
Background: The protein Nogo-A regulates axon growth in the developing and mature nervous system, and this is carried out by two distinct domains in the protein, Nogo-A-Δ20 and Nogo-66. The differences in the signalling pathways engaged in axon growth cones by these domains are not well characterized, and have been investigated in this study. Methodology/Principal Findings: We analyzed growth cone collapse induced by the Nogo-A domains Nogo-A-Δ20 and Nogo-66 using explanted chick dorsal root ganglion neurons growing on laminin/poly-lysine substratum. Collapse induced by purified Nogo-A-Δ20 peptide is dependent on protein synthesis whereas that induced by Nogo-66 peptide is not. Nogo-A-Δ20-induced collapse is accompanied by a protein synthesis-dependent rise in RhoA expression in the growth cone, but is unaffected by proteasomal catalytic site inhibition. Conversely Nogo-66-induced collapse is inhibited ∼50% by proteasomal catalytic site inhibition. Conclusion/Significance: Growth cone collapse induced by the Nogo-A domains Nogo-A-Δ20 and Nogo-66 is mediated by signalling pathways with distinguishable characteristics concerning their dependence on protein synthesis and proteasomal function. [ABSTRACT FROM AUTHOR]
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