Two additional 5' exons in the human Vigilin gene distinguish it from the chicken gene and provide the structural basis for differential routes of gene expression.

Publisher: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Country of Publication: England NLM ID: 0107600 Publication Model: Print Cited Medium: Print ISSN: 0014-2956 (Print) Linking ISSN: 00142956 NLM ISO Abbreviation: Eur J Biochem Subsets: MEDLINE

Publication: -2004: Oxford, UK : Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Original Publication: Berlin, New York, Springer.

Carrier Proteins* ; RNA-Binding Proteins*; Chickens/*genetics ; Exons/*genetics ; Gene Expression Regulation/*genetics ; Proteins/*genetics; Alternative Splicing/genetics ; Animals ; Base Sequence ; Blotting, Northern ; Cloning, Molecular ; Codon, Initiator/genetics ; DNA Primers ; Humans ; Introns/genetics ; Molecular Sequence Data ; Open Reading Frames/genetics ; Promoter Regions, Genetic/genetics ; Restriction Mapping

Vigilin, a 150-kDa protein, contains 14 tandemly arranged domains, each consisting of a KH RNA-binding motif and a spacer region. Here, we report on the physical structure of the human Vigilin gene with 29 exons, thereby outnumbering the chicken gene by two additional 5' exons. These additional exons, 1A and 1B, are alternatively though concurrently spliced to exon 1C which is homologous to the first exon in the chicken gene. None of the additional human exons code for an amino-terminal extension of Vigilin, due to in-frame stop codons. Structural features of exon 1A, however, would allow the translation of a 13-amino-acid peptide from an upstream open reading frame preceding the vigilin open reading frame. We suggest that exons 1A and 1B have been gained during evolution, allowing alternative routes of expression control of the human Vigilin gene.

0 (Carrier Proteins)
0 (Codon, Initiator)
0 (DNA Primers)
0 (Proteins)
0 (RNA-Binding Proteins)
147605-06-9 (high density lipoprotein binding protein)

Date Created: 19960601 Date Completed: 19960822 Latest Revision: 20190620

20231215

10.1111/j.1432-1033.1996.0410z.x

8681952