α-Tocopherol levels in natural and artificial aging of soybean seeds.

Teores de α-tocoferol no envelhecimento natural e artificial de sementes de soja. (Portuguese)

Tocopherols are well known constituents of vitamin E, and the main antioxidants in soybean. Are natural antioxidants and stabilizers that can inhibit lipid degradation, reducing non-enzymatic oxidation of these compounds during storage of seeds, germination and initial development of seedlings. The objective of this work was to determine the level of α-tocopherol in four soybean seeds cultivars naturally and artificially aged. Seeds of four soybean cultivars stored from 0 to 180 days in a dry chamber (natural aging) and subjected to high temperature and humidity (artificial aging) were analyzed for α-tocopherol content. The quantification of α-tocopherol in the soybean seeds was performed by high performance liquid chromatography (HPLC) combined with the Soxhlet extraction method. Significant differences in α-tocopherol levels in seeds were observed for all cultivars and storage times. The α-tocopherol contents of the soybean seeds showed linear correlations with an increasing period of storage for all the cultivars studied. However, the artificially aged seeds had a higher content of α-tocopherol than those naturally aged. [ABSTRACT FROM AUTHOR]

Tocoferóis são conhecidos constituintes da vitamina E, sendo os principais antioxidantes lipofílicos na soja. Eles são substâncias antioxidantes naturais e estabilizadoras, capazes de inibir a degradação de lipídios limitando a oxidação não enzimática destes compostos durante o armazenamento das sementes, a germinação e o desenvolvimento inicial das plântulas. O objetivo deste trabalho foi determinar o conteúdo de α-tocoferol nas sementes de quarto cultivares de soja envelhecidas natural e artificialmente. As sementes de quatro cultivares de soja envelhecidas em armazenamento de zero a 180 dias em câmara seca (envelhecimento natural) e submetidas a altas temperaturas e umidade relativa (envelhecimento artificial) foram analisadas quanto ao teor de α-tocoferol. A quantificação de á-tocoferol nas sementes de soja foi efetuada por cromatografia líquida de alta eficiência (CLAE) associada ao método de extração Soxhlet. Diferenças significativas nos teores de á-tocoferol nas sementes foram observadas para todas as cultivares e tempos de armazenamento. O teor de α-tocoferol das sementes de soja mostrou resposta linear crescente com o aumento dos períodos de armazenamento. Entretanto, as sementes envelhecidas artificialmente apresentaram maior teor de α-tocoferol do que as envelhecidas naturalmente. [ABSTRACT FROM AUTHOR]

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