Type 1 prophospholipase A2 propeptide in acute lung injury.

Publisher: Elsevier Country of Publication: England NLM ID: 2985213R Publication Model: Print Cited Medium: Print ISSN: 0140-6736 (Print) Linking ISSN: 01406736 NLM ISO Abbreviation: Lancet Subsets: MEDLINE

Publication: 2004- : London : Elsevier
Original Publication: London : J. Onwhyn

Enzyme Precursors*; Lung Diseases/*enzymology ; Phospholipases A/*blood ; Protein Precursors/*blood; Amino Acid Sequence ; Biomarkers ; Enzyme-Linked Immunosorbent Assay ; Humans ; Inflammation Mediators/blood ; Inflammation Mediators/metabolism ; Lung Diseases/immunology ; Molecular Sequence Data ; Neutrophils/immunology ; Phospholipases A/metabolism ; Phospholipases A/urine ; Phospholipases A2 ; Protein Precursors/metabolism ; Protein Precursors/urine ; Sensitivity and Specificity

Neutrophil sequestration and activation in the pulmonary vasculature and interstitium are important in acute lung injury. Phospholipase A2 plays an important part in the production of potent inflammatory mediators in this syndrome. We used our ELISA for type 1 prophospholipase A2 activation peptides, which have the aminoacid sequence Asp-Ser-Gly-Ile-Ser-Pro-Arg (DSGISPR), to show that DSGISPR concentrations in plasma and urine are a sensitive and specific marker of acute lung injury in patients admitted to intensive care. The detection of DSGISPR in the plasma of 11 of 50 unselected patients had a sensitivity of 100% and a specificity of 93% for the presence or future development of acute lung injury.

0 (Biomarkers)
0 (Enzyme Precursors)
0 (Inflammation Mediators)
0 (Protein Precursors)
EC 3.1.1.32 (Phospholipases A)
EC 3.1.1.4 (Phospholipases A2)
EC 3.1.1.4 (prophospholipase A2)

Date Created: 19941126 Date Completed: 19941216 Latest Revision: 20190611

20250114

10.1016/s0140-6736(94)90291-7

7968121