Metal transport in cells: cadmium uptake by rat hepatocytes and renal cortical epithelial cells.

Publisher: National Institute of Environmental Health Sciences Country of Publication: United States NLM ID: 0330411 Publication Model: Print Cited Medium: Print ISSN: 0091-6765 (Print) Linking ISSN: 00916765 NLM ISO Abbreviation: Environ Health Perspect Subsets: MEDLINE

Original Publication: Research Triangle Park, N. C. National Institute of Environmental Health Sciences.

Cadmium/*pharmacokinetics ; Kidney Cortex/*metabolism ; Liver/*metabolism; Animals ; Biological Transport ; Cells, Cultured ; Epithelial Cells ; Epithelium/metabolism ; Kidney Cortex/cytology ; Liver/cytology ; Rats ; Rats, Sprague-Dawley

The toxic metals appear to use the transport pathways that exist for biologically essential metals. In this regard interactions between the toxic and essential metals are possible. This report summarizes recent findings on the transport of cadmium in rat hepatocytes and renal cortical epithelial cells in the presence or absence of certain essential metals. The transport of cadmium in hepatocytes does not require energy and, therefore, is not an active process. It occurs primarily (80%) by temperature-sensitive processes, i.e., ion channels and carriers, that involve interaction with sulfhydryl groups. These processes apparently exist for the transport of essential metals like copper, zinc and calcium. The remaining 20% of the cadmium in hepatocytes is transported via a temperature-insensitive process, possibly by diffusion. In comparison with the hepatocytes, a smaller fraction (30%) of the cadmium transport through the basolateral membrane and none from the apical membrane of the renal cortical epithelial cells is temperature-sensitive. Total accumulation through the basolateral membrane is about twice that through the apical membrane. A majority of the cadmium transport in the renal cells is by diffusion. As in hepatocytes, copper, zinc and mercury antagonize cadmium transport through the apical membranes of the renal cells. The relative antagonism by copper is the same (25%); however, the antagonism by zinc (16%) and mercury (10%) is 4- to 6-fold lower than in hepatocytes. It appears that the relative contribution of various transport pathways available for cadmium uptake is different in each cell type and apparently depends on the morphological and functional differences between the cell membranes.

Am J Physiol. 1979 Mar;236(3):C139-43. (PMID: 426045)
Am J Physiol. 1983 Feb;244(2):G183-91. (PMID: 6824089)
Mol Pharmacol. 1984 Jul;26(1):75-82. (PMID: 6087119)
Biochim Biophys Acta. 1987 Aug 20;902(2):247-52. (PMID: 2956989)
J Biol Chem. 1988 Jun 25;263(18):8844-50. (PMID: 3379048)
Toxicol Appl Pharmacol. 1993 Aug;121(2):203-9. (PMID: 8346537)
J Biol Chem. 1989 Apr 5;264(10):5613-5. (PMID: 2522437)
Proc Soc Exp Biol Med. 1991 Feb;196(2):130-40. (PMID: 1990402)
Biochem Pharmacol. 1991 Mar 1;41(5):775-80. (PMID: 1998532)
Toxicol Appl Pharmacol. 1991 Sep 1;110(2):355-63. (PMID: 1654000)
Toxicol Appl Pharmacol. 1992 Mar;113(1):118-25. (PMID: 1553744)
J Cell Physiol. 1988 Aug;136(2):373-8. (PMID: 3410885)

ES-03187 United States ES NIEHS NIH HHS

00BH33GNGH (Cadmium)

Date Created: 19950201 Date Completed: 19950831 Latest Revision: 20181113

20221213

PMC1519319

10.1289/ehp.95103s173

7621805