Detection and characterisation of two novel vitiviruses infecting Actinidia.

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المؤلفون: Blouin, Arnaud¹ ; Chavan, Ramesh²; Pearson, Michael²; MacDiarmid, Robin; Cohen, Daniel¹
المصدر:
Archives of Virology. Apr2012, Vol. 157 Issue 4, p713-722. 10p. 1 Color Photograph, 2 Diagrams, 1 Chart.
الموضوع:
*PLANT viruses; *KIWIFRUIT; *VIRAL genomes; *OPEN reading frames (Genetics); *COAT proteins (Viruses); *AMINO acids; *VIRUSES

معلومة اضافية
- نبذة مختصرة :
  Two co-infecting novel vitiviruses from Actinidia chinensis were identified from mechanically inoculated Nicotiana occidentalis. Both virus genomes were sequenced and share 64% nucleotide identity. Their overall structure is typical of vitiviruses, with five open reading frames (ORFs) and a polyadenylated 3′ end. Open reading frame 4 (ORF4) encodes the coat protein, the most conserved gene of the vitiviruses, in which they share 75% amino acid identity, 61-68% with grapevine virus B, 55-59% with grapevine virus A, and 37-42% with grapevine virus E. Based on the molecular criteria for species demarcation in the family Betaflexiviridae, these are two novel viruses, tentatively named Actinidia virus A and Actinidia virus B. [ABSTRACT FROM AUTHOR]