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Effect of reagins and allergen extracts on radioallergosorbent assays for mite allergen.

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  • المؤلفون: Tovey ER; Vandenberg RA
  • المصدر:
    Clinical allergy [Clin Allergy] 1978 Jul; Vol. 8 (4), pp. 329-39.
  • نوع النشر :
    Journal Article
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 0311172 Publication Model: Print Cited Medium: Print ISSN: 0009-9090 (Print) Linking ISSN: 00099090 NLM ISO Abbreviation: Clin Allergy Subsets: MEDLINE
    • بيانات النشر:
      Publication: Oxford : Blackwell Scientific Publications
      Original Publication: Oxford, Blackwell Scientific Publications.
    • الموضوع:
      Antibodies* ; Reagins*; Allergens/*immunology ; Mites/*immunology; Binding Sites ; Binding, Competitive ; Humans ; Radioallergosorbent Test ; Serum Albumin, Bovine/pharmacology
    • نبذة مختصرة :
      The reproducibility of the radioallergosorbent (RAST) inhibition and direct binding assys with mite allergen were investigated in the presence of heterogeneous extracts and non-mite-sensitive atopic sera. Both contain components similar to potential contaminants which would occur in the assay of mite allergen and dust allergen and dust allergen extracts. The standardized inhibition and direct binding assays employed had a day to day (n = 4) coefficient of variation [(s.d. x 100)/mean] of 15% and 24% respectively. The inhibition assay for mite allergen was reproducible in the presence of protein concentrations of added plant, fungal, arthropod and animal extracts in excess of the protein concentrations that occur under the operational mite assay conditions. The mite inhibition assay was also reproducible in the presence of non-mite allergen extracts, with and without additional sera containing IgE specific for the non-mite allergens. The binding of a additional sera containing IgE specific for the non-mite allergens. The binding of a constant quantity of mite allergen to the activated solid phase in the direct binding assay was reproducible in the presence of added bovine serum albumin, and of a fungal or arthropod extract, representing the heterogeneous components of an allergen extract at the concentrations of total protein known to occur in the direct binding assay of mite extracts.
    • الرقم المعرف:
      0 (Allergens)
      0 (Antibodies)
      0 (Reagins)
      27432CM55Q (Serum Albumin, Bovine)
    • الموضوع:
      Date Created: 19780701 Date Completed: 19790115 Latest Revision: 20191021
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1111/j.1365-2222.1978.tb00468.x
    • الرقم المعرف:
      709785