Flow Cytometry: Principles, Methodology and Applications

Flow cytometry is a method to conduct a multiparameter analysis of cells suspended in liquid and passing through a laser beam. As cells pass through the flow chamber, they are hit with a laser light beam which is scattered in different directions, and recorded as forward light-scatter and side light-scatter. In this book, the authors discuss the principles, methodology and applications of flow cytometry. Topics include flow cytometry and epifluorescence analyses of freshwater bacterio- and virioplankton communities; application of flow cytometry in phenotyping, analysis and functional characterization of T regulatory and immune suppressor cells; multicolor panel design strategies in rare event analysis of dendritic cells and regulatory T cells; advanced characterization of individual particle morphology light-scattering flow cytometry; and application of flow cytometry in environmental science.