Escherichia coli type I toxin TisB exclusively controls proton depolarization following antibiotic induced DNA damage.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

DNA Damage*/drug effects ; Anti-Bacterial Agents*/pharmacology ; Escherichia coli*/drug effects ; Escherichia coli*/metabolism ; Escherichia coli*/genetics ; Escherichia coli Proteins*/metabolism ; Escherichia coli Proteins*/genetics ; Protons* ; Bacterial Toxins*/metabolism ; Bacterial Toxins*/genetics; Ciprofloxacin/pharmacology ; Adenosine Triphosphate/metabolism ; Toxin-Antitoxin Systems

Competing Interests: Delcaration. Competing interests: The authors declare that they have no competing interests.
Bacterial toxin-antitoxin (TA) systems are genetic loci where the antitoxin gene product helps to control the expression or activity of the toxin gene product. Type I TA systems typically produce hydrophobic peptides that often localize to the inner membrane of bacteria. These amphipathic peptides can then potentially affect ion flows across the inner membrane. Here, we show that several type I toxins from Escherichia coli can affect depolarization, whereas tisB exclusively controls the depolarization of the proton gradient. tisB has been linked to persister cell formation following treatment with the antibiotic ciprofloxacin and tisB-istR has been implicated in the control of proton depolarization following treatment with ofloxacin. These results suggest that tisB could initiate the formation of persister cells by fully dissipating the proton gradient and that most of the electrical gradient greatly limiting ATP production following antibiotic-induced DNA damage.
(© 2025. The Author(s).)

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Keywords: E. coli; Antibiotics; DNA damage; Membrane depolarization; TisB; Type I toxin antitoxin systems

0 (Anti-Bacterial Agents)
0 (Escherichia coli Proteins)
0 (Protons)
0 (Bacterial Toxins)
5E8K9I0O4U (Ciprofloxacin)
8L70Q75FXE (Adenosine Triphosphate)

Date Created: 20250414 Date Completed: 20250414 Latest Revision: 20250417

20250417

PMC11997105

10.1038/s41598-025-96136-x

40229382