Systematic identification of R2R3-MYB S6 subfamily genes in Brassicaceae and its role in anthocyanin biosynthesis in Brassica crops.

Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Anthocyanins*/biosynthesis ; Anthocyanins*/genetics ; Phylogeny* ; Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Transcription Factors*/genetics ; Transcription Factors*/metabolism; Crops, Agricultural/genetics ; Crops, Agricultural/metabolism ; Brassicaceae/genetics ; Brassicaceae/metabolism ; Gene Expression Regulation, Plant ; Brassica/genetics ; Brassica/metabolism ; Genes, Plant

The Brassicaceae family includes Arabidopsis thaliana, various vegetables and oil crops. The R2R3-MYB genes of the S6 subfamily are crucial for regulating anthocyanin biosynthesis, however, their systematic identification in Brassicaceae plants is still incomplete. Here, we systematically identified homologous genes of R2R3-MYB transcription factors from the S6 subfamily across 31 Brassicaceae species. A total of 92 homologous genes were identified, with species representation ranging from 0 to 10 genes per species. Phylogenetic analysis classified these homologous genes into six distinct groups. Notably, approximately 70% of the homologous genes were found within the G6 group, indicating a high degree of evolutionary conservation. Furthermore, a phylogenetic analysis was conducted on 35 homologous genes obtained from six species within the U's triangle Brassica plants. The findings provided evidence of significant conservation among orthologous genes across species and demonstrated strong collinearity on subgenomic chromosomes, with notable tandem duplications observed on chromosomes A7 and C6. Subsequently, we predicted the cis-acting elements of these 35 homologous genes, and analyzed their structures, conserved motifs, and characteristic conserved domains, confirming the significant similarities between orthologous genes. Additionally, we employed white and purple flower rapeseed specimens to conduct qRT-PCR validation of the key genes and transcriptional regulators associated with the anthocyanin synthesis pathway. The results revealed significant differential expression of BnaPAP2.A7.b in purple flowers, alongside the differential expression of BnaPAP2.C6.d. Ultimately, based on previous research and the findings of this study, we propose a transcriptional regulatory framework to govern anthocyanin accumulation in distinct tissues or organs of B. napus. Our findings offer a novel perspective on the functional diversification of R2R3-MYB transcription factors within the S6 subfamily homologous genes, while also shedding light on the regulatory network governing anthocyanin biosynthesis in Brassicaceae species.
Competing Interests: Declarations. Ethics approval and consent to participate: This study including the collection on plants material complies with relevant institutional, national, and international guidelines and legislation. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
(© 2025. The Author(s).)

Nature. 2000 Dec 14;408(6814):796-815. (PMID: 11130711)
Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18724-8. (PMID: 20921408)
BMC Genomics. 2022 Mar 19;23(1):220. (PMID: 35305581)
Mol Plant. 2015 May;8(5):689-708. (PMID: 25840349)
J Exp Bot. 2022 Nov 2;73(19):6630-6645. (PMID: 35857343)
Plant Physiol. 2022 Aug 29;190(1):226-237. (PMID: 35670735)
Int J Mol Sci. 2021 Jun 07;22(11):. (PMID: 34200125)
Plant Sci. 2023 Oct;335:111811. (PMID: 37574139)
Plant Mol Biol. 2022 Sep;110(1-2):53-68. (PMID: 35723867)
Curr Opin Plant Biol. 2014 Jun;19:81-90. (PMID: 24907528)
Nat Plants. 2020 Aug;6(8):929-941. (PMID: 32782408)
Curr Opin Plant Biol. 2001 Oct;4(5):447-56. (PMID: 11597504)
Theor Appl Genet. 2019 Apr;132(4):895-906. (PMID: 30467611)
Crit Rev Food Sci Nutr. 2022;62(8):2205-2220. (PMID: 33256437)
New Phytol. 2019 May;222(3):1638-1651. (PMID: 30735246)
Recent Pat Biotechnol. 2014;8(1):47-60. (PMID: 24354533)
BMC Genomics. 2022 Jun 14;23(1):441. (PMID: 35701743)
Plant Physiol. 2012 Jun;159(2):531-47. (PMID: 22529285)
Plant J. 2023 Mar;113(5):1062-1079. (PMID: 36606413)
Nat Genet. 2021 Sep;53(9):1392-1402. (PMID: 34493868)
Trends Plant Sci. 2010 Oct;15(10):573-81. (PMID: 20674465)
Plant Physiol. 2021 May 27;186(1):388-406. (PMID: 33599732)
Plant J. 2011 Apr;66(1):94-116. (PMID: 21443626)
Appl Microbiol Biotechnol. 2022 Mar;106(5-6):1783-1798. (PMID: 35171341)
Int J Mol Sci. 2022 Oct 02;23(19):. (PMID: 36233003)
Theor Appl Genet. 2009 Dec;120(1):45-57. (PMID: 19779693)
J Exp Bot. 2017 Jul 10;68(15):4013-4028. (PMID: 28922752)
Nat Plants. 2021 Jun;7(6):757-765. (PMID: 34045706)
Front Plant Sci. 2020 Aug 19;11:983. (PMID: 32973819)
Hortic Res. 2022 Jan 19;:. (PMID: 35043168)
Bioinformatics. 2019 Nov 1;35(21):4453-4455. (PMID: 31070718)
Nucleic Acids Res. 2021 Jul 2;49(W1):W293-W296. (PMID: 33885785)
Hortic Res. 2020 Feb 21;7:20. (PMID: 32133148)
Nat Genet. 2011 Aug 28;43(10):1035-9. (PMID: 21873998)
Mol Plant. 2023 Nov 6;16(11):1733-1742. (PMID: 37740491)
Funct Integr Genomics. 2013 Mar;13(1):75-98. (PMID: 23184474)
Hortic Res. 2022 Mar 08;9:uhac058. (PMID: 35591925)
Mol Breed. 2023 Mar 9;43(3):19. (PMID: 37313299)
New Phytol. 2014 Apr;202(1):132-144. (PMID: 24299194)
Plant Cell. 1999 Aug;11(8):1433-44. (PMID: 10449578)
Science. 2014 Aug 22;345(6199):950-3. (PMID: 25146293)
Hortic Res. 2020 Jul 1;7:97. (PMID: 32637125)
Mol Biol Evol. 2020 May 1;37(5):1530-1534. (PMID: 32011700)
BMC Genomics. 2023 Mar 9;24(1):103. (PMID: 36894869)
Nat Commun. 2014 May 23;5:3930. (PMID: 24852848)
Theor Appl Genet. 2020 Nov;133(11):2989-3000. (PMID: 32870325)
BMC Plant Biol. 2021 Jun 29;21(1):301. (PMID: 34187365)
Plant Cell. 2006 Jun;18(6):1348-59. (PMID: 16632643)
Plant Physiol. 2017 Aug;174(4):2062-2071. (PMID: 28667048)

20232BCJ23089 the Ganpo Talent Support Program of Jiangxi Province; 20232BCJ23089 the Ganpo Talent Support Program of Jiangxi Province; 20232BCJ23089 the Ganpo Talent Support Program of Jiangxi Province; 20232BCJ23089 the Ganpo Talent Support Program of Jiangxi Province; 20232BCJ23089 the Ganpo Talent Support Program of Jiangxi Province; 20223BBF61002 the Key Research and Development Program of Jiangxi Province; 20223BBF61002 the Key Research and Development Program of Jiangxi Province; 20223BBF61002 the Key Research and Development Program of Jiangxi Province; 20223BBF61002 the Key Research and Development Program of Jiangxi Province; 20223BBF61002 the Key Research and Development Program of Jiangxi Province; 20223BBF61002 the Key Research and Development Program of Jiangxi Province; 20204BCJL22046 Jiangxi Province's training program for academic and technical leaders in major disciplines

Keywords: Brassica napus; Anthocyanin biosynthesis; Brassicaceae; Homologous gene; R2R3-MYB S6 subfamily genes

0 (Anthocyanins)
0 (Plant Proteins)
0 (Transcription Factors)

Date Created: 20250305 Date Completed: 20250306 Latest Revision: 20250308

20250308

PMC11883967

10.1186/s12870-025-06296-1

40045187