Therapeutic anti-inflammatory immune potentials of some seaweeds extracts on chemically induced liver injury in mice.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Chemical and Drug Induced Liver Injury*/drug therapy ; Chemical and Drug Induced Liver Injury*/pathology ; Chemical and Drug Induced Liver Injury*/metabolism ; Anti-Inflammatory Agents*/pharmacology ; Anti-Inflammatory Agents*/therapeutic use ; Seaweed*/chemistry ; Carbon Tetrachloride*/toxicity ; Plant Extracts*/pharmacology ; Plant Extracts*/chemistry; Animals ; Mice ; Liver/drug effects ; Liver/pathology ; Liver/metabolism ; Liver/injuries ; Male ; Alanine Transaminase/blood ; Cytokines/metabolism

Carbon tetrachloride (CCl 4 ) is a well-known hepatotoxin. This work aimed to assess the therapeutic anti-inflammatory immune potentials of the seaweeds Padina pavonia and Jania rubens extracts on carbon tetrachloride (CCL 4 )-caused liver damage in mice. Our experimentation included two testing regimens: pre-treatment and post-treatment of P. pavonia and J. rubens extracts in CCL 4 /mice. Pre-treatment and post-treatment of P. pavonia and J. rubens extracts in CCL 4 /mice increased WBCs count and lymphocytes relative numbers and reduced the neutrophils and monocytes relative numbers. Pre-treatment and post-treatment of CCL4 /mice with P. pavonia and J. rubens extracts significantly reduced the release amounts of pro-inflammatory cytokines TNF-α and IL-6 and significantly inhibited the increased CRP level. Furthermore, pre-treatment and post-treatment of CCL 4 /mice with P. pavonia and J. rubens extracts recovered the activities of GSH, and significantly decreased MDA level. CCL4/mice pre-treated and post-treated with P. pavonia and J. rubens extracts decreased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. Pre- and post-treatment of CCL 4 /mice with the P. pavonia and J. rubens extracts ameliorated the liver damages caused by CCl 4 and significantly inhibited the necrotic area, indicating hepatic cell death and decreased periportal hepatic degeneration, fibrosis, and inflammation.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Ethical approval and consent to participate: All animal experimentation protocols were carried out in agreement with the National Institutes of Health guide for the care and use of laboratory animals (NIH Publications No. 8023, revised 1978) and following the recommendation of the proper care and use of laboratory animals regulated by Faculty of Science, Tanta University IACUC, Tanta, Egypt. This study was approved by the Faculty of Science, Tanta University’s Scientific Induction Ethics Committee, and guidelines for the humane care of animals (IACUC-SCI–TU-0412). It is also confirmed that this study is reported in accordance with ARRIVE guidelines. Consent to publish: The authors agreed to publish this manuscript for publication.
(© 2025. The Author(s).)

Mol Immunol. 2017 May;85:222-229. (PMID: 28314211)
Gastroenterology. 2008 May;134(6):1655-69. (PMID: 18471545)
Int J Biol Macromol. 2019 Jun 15;131:614-623. (PMID: 30898597)
Circ Res. 2012 Feb 3;110(3):416-27. (PMID: 22194622)
J Hazard Mater. 2011 Nov 15;195:208-13. (PMID: 21880419)
J Clin Invest. 2005 Feb;115(2):209-18. (PMID: 15690074)
Mediators Inflamm. 1997;6(3):201-10. (PMID: 18472821)
Oxid Med Cell Longev. 2018 Aug 1;2018:9354296. (PMID: 30154951)
Immunol Rev. 2004 Dec;202:275-93. (PMID: 15546400)
J Pharmacol Exp Ther. 1969 Mar;166(1):96-103. (PMID: 5776026)
Annu Rev Immunol. 1985;3:263-87. (PMID: 2933048)
Annu Rev Pharmacol Toxicol. 1995;35:655-77. (PMID: 7598511)
Am J Chin Med. 2011;39(1):15-27. (PMID: 21213395)
Can J Physiol Pharmacol. 2015 Jan;93(1):39-43. (PMID: 25415237)
Int J Biochem Cell Biol. 2016 Dec;81(Pt B):364-374. (PMID: 27686849)
Int Immunopharmacol. 2017 Feb;43:91-98. (PMID: 27987467)
Evid Based Complement Alternat Med. 2011;2011:673083. (PMID: 21860651)
J Neuroimmune Pharmacol. 2010 Mar;5(1):83-91. (PMID: 20020329)
Oxid Med Cell Longev. 2017;2017:3140673. (PMID: 28203318)
J Antibiot (Tokyo). 2019 Jun;72(6):350-363. (PMID: 30911163)
Hepatology. 2009 Jul;50(1):185-97. (PMID: 19441102)
Acta Gastroenterol Belg. 2011 Dec;74(4):548-52. (PMID: 22319965)
Biol Trace Elem Res. 2017 Mar;176(1):154-175. (PMID: 27498811)
Food Chem Toxicol. 2001 Feb;39(2):119-24. (PMID: 11267704)
Cancer Chemother Pharmacol. 2005 Oct;56(4):436-46. (PMID: 15902462)
J Clin Transl Res. 2017 Feb;3(Suppl 1):170-188. (PMID: 28670626)
Phytother Res. 1999 Mar;13(2):120-3. (PMID: 10190184)
J Clin Invest. 2002 Sep;110(5):651-8. (PMID: 12208866)
Pak J Biol Sci. 2007 Feb 15;10(4):617-21. (PMID: 19069545)
Virol J. 2008 Jan 15;5:8. (PMID: 18197976)
Curr Drug Targets Inflamm Allergy. 2005 Jun;4(3):281-6. (PMID: 16101534)
Am J Chin Med. 1984 Summer;12(1-4):106-14. (PMID: 6208771)
Blood. 1997 Oct 1;90(7):2772-83. (PMID: 9326245)
Pharm Biol. 2014 Dec;52(12):1581-90. (PMID: 25243881)
Biotechnol Appl Biochem. 2007 Jan;46(Pt 1):69-72. (PMID: 16984227)
Respir Res. 2002;3:5. (PMID: 11806840)
Crit Rev Toxicol. 2003;33(2):105-36. (PMID: 12708612)
Hepatology. 2004 Jun;39(6):1631-8. (PMID: 15185304)
J Ethnopharmacol. 2008 Feb 28;116(1):187-90. (PMID: 18079077)
Curr Opin Toxicol. 2018 Feb;7:81-86. (PMID: 29862377)
Free Radic Biol Med. 1996;20(3):285-90. (PMID: 8720898)
Semin Liver Dis. 2019 May;39(2):111-123. (PMID: 30912097)
Toxicol Appl Pharmacol. 1993 Apr;119(2):275-9. (PMID: 8480336)
Nutrients. 2021 Jul 29;13(8):. (PMID: 34444774)
Molecules. 2022 Sep 16;27(18):. (PMID: 36144768)
Biochem J. 1971 Aug;123(5):805-14. (PMID: 4399399)
Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2575-80. (PMID: 9122237)
Front Biosci. 2003 Jan 01;8:d69-77. (PMID: 12456323)
Food Chem Toxicol. 2018 Oct;120:491-499. (PMID: 30056145)
Biochimie. 2011 May;93(5):806-16. (PMID: 21277932)
Drug Metab Rev. 1995;27(1-2):73-92. (PMID: 7641586)
Eur J Clin Invest. 2008 Jul;38(7):523-30. (PMID: 18578693)
J Hepatol. 2017 Jun;66(6):1300-1312. (PMID: 28267621)
Cytokine Growth Factor Rev. 2009 Apr;20(2):97-113. (PMID: 19268625)
Kidney Int Suppl. 2005 Dec;(99):S98-102. (PMID: 16336586)
Int J Biol Macromol. 2018 Feb;107(Pt A):803-807. (PMID: 28962845)
Nature. 2011 Jun 08;475(7355):222-5. (PMID: 21654748)
Comp Biochem Physiol B. 1993 Nov;106(3):477-87. (PMID: 8281748)
Oxid Med Cell Longev. 2015;2015:610813. (PMID: 25834699)
Carbohydr Polym. 2019 May 15;212:89-101. (PMID: 30832884)
Mol Cell Biochem. 2009 Jul;327(1-2):219-28. (PMID: 19242656)
Fed Proc. 1987 Jan;46(1):118-26. (PMID: 3100339)
Cancer Cell Int. 2022 Aug 23;22(1):267. (PMID: 35999584)
J Ethnopharmacol. 2013 Dec 12;150(3):973-81. (PMID: 24140589)
Pak J Pharm Sci. 2017 May;30(3):721-728. (PMID: 28653915)
Future Med Chem. 2011 Sep;3(12):1475-89. (PMID: 21882941)
J Surg Res. 2007 Apr;138(2):209-13. (PMID: 17291534)
Oxid Med Cell Longev. 2018 Dec 3;2018:3894381. (PMID: 30622666)
Fish Shellfish Immunol. 2006 Mar;20(3):332-45. (PMID: 16005643)
Am J Pathol. 1994 Nov;145(5):1191-8. (PMID: 7977649)
Cell Mol Immunol. 2014 May;11(3):263-74. (PMID: 24561457)
Phytomedicine. 2000 Mar;7(1):1-6. (PMID: 10782483)
Adv Exp Med Biol. 2012;739:237-51. (PMID: 22399406)
Biochem Med (Zagreb). 2012;22(1):49-62. (PMID: 22384519)
Nutr Metab (Lond). 2013 Feb 03;10(1):20. (PMID: 23374533)
J Biol Chem. 2004 Nov 19;279(47):48487-90. (PMID: 15337754)
Chem Biol Interact. 2007 Jan 5;165(1):76-85. (PMID: 17157831)
Clin Exp Pharmacol Physiol. 2008 Mar;35(3):303-9. (PMID: 17973934)
Nat Med. 2000 May;6(5):564-7. (PMID: 10802713)

Keywords: Anti-inflammatory; Antioxidants; Hepatoprotective; Immunomodulatory; Seaweeds

0 (Anti-Inflammatory Agents)
CL2T97X0V0 (Carbon Tetrachloride)
0 (Plant Extracts)
EC 2.6.1.2 (Alanine Transaminase)
0 (Cytokines)

Date Created: 20250205 Date Completed: 20250205 Latest Revision: 20250208

20250208

PMC11799325

10.1038/s41598-025-87379-9

39910080