ρ-Coumaric acid-zinc oxide nanoparticles improve post-thaw quality of goat spermatozoa and developmental competence of fertilized oocytes in vitro.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Goats* ; Zinc Oxide*/pharmacology ; Zinc Oxide*/chemistry ; Cryopreservation*/methods ; Spermatozoa*/drug effects ; Coumaric Acids*/pharmacology ; Sperm Motility*/drug effects ; Fertilization in Vitro*/methods ; Fertilization in Vitro*/veterinary; Animals ; Male ; Female ; Oocytes/drug effects ; Oocytes/metabolism ; Semen Preservation/methods ; Semen Preservation/veterinary ; Nanoparticles/chemistry ; Reactive Oxygen Species/metabolism ; Antioxidants/pharmacology

Excessive production of reactive oxygen species (ROS) during cryopreservation and post-thawing affects sperm quality and subsequent fertilizing capacity. Nanoparticles (NPs) with antioxidative properties can improve sperm function and male fertility. The aim of this study was to assess the effect of 100 µM ρ-coumaric acid (ρ-CA), 0.1 µM ρ-CA-NPs (PCNPs), 150 µg/mL zinc chloride (ZnCl 2 ), 1 µg/mL zinc oxide-NPs (ZnO-NPs), ρ-CA + ZnCl 2 , PCNPs + ZnO-NPs, 0.001 µM of ρ-CA loaded on ZnO-NPs (ρ-CA-ZnONPs) on goat sperm parameters and fertilizing ability after cryopreservation. Semen samples from five Saanen goats were used. Various concentrations of treatments were incubated to determine the optimal concentrations for assessing sperm motility and viability. Subsequently, samples were filled with 0.5-mL straws, frozen, and stored in liquid nitrogen (- 196 °C). Evaluations of post-thaw spermatozoa parameters and fertilizing ability were performed. Addition of ρ-CA-ZnONPs and PCNPs + ZnO-NPs significantly increased sperm viability, motility, plasma membrane integrity, blastocyst rate, and blastocyst quality compared with the other treatments. Moreover, using ρ-CA-ZnONPs significantly decreased lipid peroxidation and DNA damage compared with the other treatments. In conclusion, spermatozoa are cryotolerant, resistant to post-thaw conditions, and have fertilizing ability that can be increased by adding ρ-CA-ZnONPs as an antioxidant to goat semen extenders.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)

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Keywords: Cryopreservation; Goat; Nanoparticles; Sperm; Zinc oxide; ρ-Coumaric acid

SOI2LOH54Z (Zinc Oxide)
0 (Coumaric Acids)
0 (Reactive Oxygen Species)
0 (Antioxidants)

Date Created: 20241231 Date Completed: 20241231 Latest Revision: 20250104

20250104

PMC11686304

10.1038/s41598-024-83585-z

39738447