Genotypic diversity and virulence factors of Klebsiella pneumoniae in a North Indian tertiary care hospital.

Publisher: BioMed Central Country of Publication: England NLM ID: 100968551 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2334 (Electronic) Linking ISSN: 14712334 NLM ISO Abbreviation: BMC Infect Dis Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Klebsiella pneumoniae*/genetics ; Klebsiella pneumoniae*/pathogenicity ; Klebsiella pneumoniae*/classification ; Klebsiella pneumoniae*/isolation & purification ; Tertiary Care Centers* ; Virulence Factors*/genetics ; Klebsiella Infections*/microbiology ; Klebsiella Infections*/epidemiology ; Genotype* ; Genetic Variation* ; Multilocus Sequence Typing*; Humans ; India/epidemiology ; Female ; Male ; Middle Aged ; Adult ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Virulence/genetics ; Bacterial Proteins/genetics ; Microbial Sensitivity Tests ; Biofilms/growth & development ; Aged

Klebsiella pneumoniae, a pathogen of concern worldwide can be classified as classical K. pneumoniae (cKp) and Hypervirulent K. pneumoniae (HvKp). In health care settings, genotyping and identification of hypervirulent strains enables better clinical management. The present study was conducted in a north Indian tertiary care hospital which receives both Indian and foreign patients. During the study period (September -December 2022), 29 isolates of K. pneumoniae were collected from Out Patients department (OPD), Intensive Care Unit (ICU) and wards. Genotypic profiling was based on Multi Locus Sequence Typing (MLST) and wzi type. Virulence was determined by PCR based screening of genes (RmpA, iucB, ybts, iroN) as well as phenotypic tests: string test, biofilm formation and serum neutralization assay. We identified 17 Sequence Type and 14 wzi strain. The most common Sequence Type was ST231 (6/29) followed by ST 6260 (3/29)0.15 (51.7%) isolates were carbapenem resistant (CR). Eleven isolates carried the aerobactin gene iucB and/or rmpA genes. The results of the study show the presence of diverse genotypes and virulence genes of K. pneumoniae, stressing the need for stricter surveillance. We also observed a significantly higher average length of stay in patients carrying hypervirulent or carbapenem resistant strains (p < 0.05).
Competing Interests: Declarations. Ethics approval and consent to participate: The Study design was approved by the Institutional Ethics Committee of Artemis Health Institute. Individual consent was not taken as the samples were collected retrospectively and the samples consisted of discarded bacterial plates. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)

Ann Clin Microbiol Antimicrob. 2023 Jan 3;22(1):1. (PMID: 36597098)
J Antimicrob Chemother. 2010 Mar;65(3):490-5. (PMID: 20071363)
Microbiol Spectr. 2023 Mar 22;:e0260722. (PMID: 36946763)
J Clin Microbiol. 2013 Dec;51(12):4073-8. (PMID: 24088853)
PLoS Genet. 2019 Apr 15;15(4):e1008114. (PMID: 30986243)
Int J Microbiol. 2022 Aug 18;2022:4532707. (PMID: 36032181)
BMC Microbiol. 2019 Jun 24;19(1):137. (PMID: 31234800)
Int J Med Microbiol. 2024 Mar;314:151601. (PMID: 38359735)
BMC Microbiol. 2022 Mar 29;22(1):81. (PMID: 35350977)
J Antibiot (Tokyo). 2021 Jul;74(7):450-457. (PMID: 33820943)
Front Med (Lausanne). 2021 Mar 08;8:645955. (PMID: 33763435)
Clin Infect Dis. 2020 Oct 23;71(7):e141-e150. (PMID: 31712802)
J Infect Dis. 1993 Dec;168(6):1415-21. (PMID: 7902383)
Front Microbiol. 2019 Apr 02;10:542. (PMID: 31001209)
One Health. 2023 Jun 26;17:100594. (PMID: 37448770)
Appl Environ Microbiol. 2020 Mar 18;86(7):. (PMID: 32005732)
J Antimicrob Chemother. 2018 May 1;73(5):1235-1241. (PMID: 29415120)
Nat Commun. 2023 Dec 2;14(1):7962. (PMID: 38042959)
Microb Drug Resist. 2021 Aug;27(8):1096-1104. (PMID: 33720791)
Gut Pathog. 2021 Jun 22;13(1):40. (PMID: 34154656)
FEMS Microbiol Rev. 2017 May 1;41(3):252-275. (PMID: 28521338)
Sci Rep. 2017 Dec 11;7(1):17291. (PMID: 29230010)
J Clin Microbiol. 2005 Aug;43(8):4178-82. (PMID: 16081970)
Clin Microbiol Rev. 2019 May 15;32(3):. (PMID: 31092506)
J Intern Med. 2020 Mar;287(3):283-300. (PMID: 31677303)
Microbiol Res. 2015 Mar;172:68-78. (PMID: 25530579)
Access Microbiol. 2022 Feb 9;4(2):000321. (PMID: 35355869)
Acta Microbiol Immunol Hung. 2019 Sep 1;66(3):367-376. (PMID: 31438725)
Front Microbiol. 2022 Feb 10;13:838207. (PMID: 35222344)
Infect Drug Resist. 2018 Jul 31;11:1031-1041. (PMID: 30104891)

Keywords: Klebsiella pneumoniae; Carbapenem resistance; India; Virulence

0 (Virulence Factors)
0 (Anti-Bacterial Agents)
0 (Bacterial Proteins)

Date Created: 20241228 Date Completed: 20241229 Latest Revision: 20250104

20250104

PMC11682614

10.1186/s12879-024-10012-z

39732681