Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Preferred Orientation of Hydroxyapatite Ceramics Along the c -Axis Promotes Osteoblast Differentiation.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • الموضوع:
      Osteoblasts*/cytology ; Osteoblasts*/metabolism ; Osteoblasts*/drug effects ; Cell Differentiation*/drug effects ; Ceramics*/chemistry ; Ceramics*/pharmacology ; Durapatite*/chemistry ; Osteogenesis*/drug effects; Humans ; Alkaline Phosphatase/metabolism ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Cell Line, Tumor
    • نبذة مختصرة :
      Hydroxyapatite (HAp) is similar to the main inorganic components of bone and tooth enamel. Furthermore, it possesses biocompatibility, making it suitable for clinical use in artificial bones. This study aimed to verify whether the preferred orientation of HAp influences osteogenesis. Using the templated grain growth method, we successfully fabricated HAp ceramics with a preferred orientation to m ( a )-planes (aHAp) and examined the effects of this orientation on bone differentiation. Osteosarcoma-derived osteoblasts (MG-63) were cultured on aHAp and HAp ceramics made from commercially available powder (iHAp). Electron backscatter diffraction analysis revealed the crystal orientation distribution of HAp ceramics and the numerous exposed a -planes of aHAp. The MG-63 cultured on aHAp exhibited significantly higher alkaline phosphatase activity, a marker of early bone differentiation, compared to iHAp. Furthermore, the two-dimensional electrophoresis results indicated that the expressed proteins differed between aHAp and iHAp. These results indicate that controlling HAp's crystal structure may promote the osteogenic potential of osteoblasts. In this study, we propose that the a -plane of HAp promotes bone differentiation during the early stages, presenting a promising approach for novel biomaterials, such as high-performance artificial bones.
    • References:
      Acta Biomater. 2020 Jan 1;101:544-553. (PMID: 31678741)
      Yale J Biol Med. 2014 Dec 12;87(4):549-61. (PMID: 25506287)
      Mater Today Bio. 2019 Sep 24;4:100031. (PMID: 32159156)
      Bone. 2002 Oct;31(4):479-87. (PMID: 12398943)
      Kobe J Med Sci. 2007;53(1-2):25-35. (PMID: 17579299)
      Acta Biomater. 2015 Oct;25:347-55. (PMID: 26232621)
      Cell Calcium. 2019 Nov;83:102058. (PMID: 31425929)
      Biomaterials. 2005 Aug;26(23):4847-55. (PMID: 15763264)
      Acta Biomater. 2011 Jul;7(7):2960-8. (PMID: 21421085)
      Biomaterials. 2005 Jun;26(17):3427-33. (PMID: 15621231)
      Biomaterials. 2002 Jul;23(14):2965-72. (PMID: 12069338)
      Front Bioeng Biotechnol. 2022 Mar 31;10:810880. (PMID: 35433652)
      Biomaterials. 1998 Jul;19(14):1245-53. (PMID: 9720888)
      Materials (Basel). 2015 Apr 15;8(4):1778-1816. (PMID: 28788032)
      J Mater Sci Mater Med. 2013 May;24(5):1211-6. (PMID: 23386210)
      Cell. 1977 Dec;12(4):1133-41. (PMID: 23215)
      Int J Pharm. 2021 May 1;600:120489. (PMID: 33744449)
      Int J Mol Sci. 2021 Dec 03;22(23):. (PMID: 34884885)
      Acta Biomater. 2013 May;9(5):6732-40. (PMID: 23403169)
      Sci Technol Adv Mater. 2021 Aug 16;22(1):683-694. (PMID: 34434075)
      Mater Sci Eng C Mater Biol Appl. 2013 Jul 1;33(5):2534-40. (PMID: 23623065)
      Regen Ther. 2021 Nov 11;18:472-479. (PMID: 34853808)
      Biomaterials. 2007 Jul;28(19):2923-31. (PMID: 17400290)
      Biomaterials. 2019 Jul;209:103-110. (PMID: 31030082)
      Int J Mol Sci. 2022 Aug 27;23(17):. (PMID: 36077119)
      Biol Pharm Bull. 2013;36(11):1654-61. (PMID: 24189407)
    • Grant Information:
      S1101019 Private Universities Foundation for the Development of Fundamental Research Strategies: De-velopment of Next-generation Regenerative Medicine Process Using Biomaterials with Life Function on the Basis of Vertical Integration System; Meiji University International Institute for Materials with Life Functions.
    • Contributed Indexing:
      Keywords: anisotropy control; bone differentiation; crystal plane; hydroxyapatite; osteoblast
    • الرقم المعرف:
      91D9GV0Z28 (Durapatite)
      EC 3.1.3.1 (Alkaline Phosphatase)
      0 (Biocompatible Materials)
    • الموضوع:
      Date Created: 20241217 Date Completed: 20241217 Latest Revision: 20250104
    • الموضوع:
      20250104
    • الرقم المعرف:
      PMC11640823
    • الرقم المعرف:
      10.3390/ijms252312926
    • الرقم المعرف:
      39684637