Overexpression of the Potato StPYL20 Gene Enhances Drought Resistance and Root Development in Transgenic Plants.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Solanum tuberosum*/genetics ; Solanum tuberosum*/growth & development ; Solanum tuberosum*/metabolism ; Plants, Genetically Modified*/genetics ; Gene Expression Regulation, Plant* ; Droughts* ; Plant Roots*/genetics ; Plant Roots*/growth & development ; Plant Roots*/metabolism ; Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Nicotiana*/genetics ; Nicotiana*/growth & development ; Stress, Physiological*/genetics; Abscisic Acid/metabolism ; Drought Resistance

Drought is a primary limiting factor for potato growth. PYR/PYL/RCAR (referred to hereafter as PYL) proteins, as receptors for abscisic acid (ABA), play a crucial role in the plant response to drought stress. However, the underlying mechanisms of this control remain largely elusive in potatoes. In this study, a potato StPYL20 gene was identified through genome-wide investigation and transcriptome analysis under drought stress. Molecular feature analysis revealed that the StPYL20 gene exhibits the highest expression level in tubers, and is significantly up-regulated under ABA and drought stress conditions. The StPYL20 protein harbors a conserved domain exclusive to the PYL family. Further functional analysis showed that both transient and stable expressions of StPYL20 in tobacco enhanced the drought resistance of transgenic plants, resulting in increased plant height, leaf number, and fresh weight, and an improved root system. Compared to wild-type plants under drought conditions, transgenic tobacco with the StPYL20 gene exhibited lower levels of malondialdehyde (MDA), higher proline (Pro) accumulation, and increased antioxidant enzyme activity. Moreover, overexpression of the StPYL20 gene heightened the sensitivity of transgenic plants to ABA. Furthermore, StPYL20 up-regulated the expression of stress response and development-related genes in transgenic plants under drought stress. In conclusion, our findings indicated that StPYL20 enhances drought resistance and root development in transgenic plants, and plays a positive regulatory role in the potato's response to drought stress.

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24JRRA838 the Natural Science Foundation of Gansu Province of China; 23ZYQJ304 the Central Government Guidance for Local Scientific and Technological Development Projects; GSAU-DKZY-2024-005 the China Agricultural University Corresponding Support Research Joint Fund; 23JRRA1414 the Gansu Provincial Science and Technology Department; 24JRRA638 the Gansu Provincial Science and Technology Department; 2024A-052 the Gansu Province Education Science and Technology Innovation Project; 2024CYZC-29 the University Industry Support Program

Keywords: ABA; PYR/PYL/RCAR; StPYL20; drought stress; potato

0 (Plant Proteins)
72S9A8J5GW (Abscisic Acid)

Date Created: 20241217 Date Completed: 20241217 Latest Revision: 20250104

20250104

PMC11641466

10.3390/ijms252312748

39684460