Therapeutic and Diagnostic Potential of a Novel K1 Capsule Dependent Phage, JSSK01, and Its Depolymerase in Multidrug-Resistant Escherichia coli Infections.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Escherichia coli*/genetics ; Escherichia coli*/drug effects ; Drug Resistance, Multiple, Bacterial*/genetics ; Escherichia coli Infections*/microbiology ; Escherichia coli Infections*/drug therapy ; Zebrafish*; Animals ; Bacterial Capsules/metabolism ; Bacteriophages/genetics ; Phage Therapy ; Humans ; Antigens, Bacterial ; Polysaccharides, Bacterial

Bacteriophages are viruses that have the potential to combat bacterial infections caused by antimicrobial-resistant bacterial strains. In this study, we investigated a novel lytic bacteriophage, vB_EcoS_JSSK01, isolated from sewage in Hualien, Taiwan, which effectively combats multidrug-resistant (MDR) Escherichia coli of the K1 capsular type. K1 E. coli is a major cause of severe extraintestinal infections, such as neonatal meningitis and urinary tract infections. Phage JSSK01 was found to have a genome size of 44,509 base pairs, producing approximately 123 particles per infected cell in 35 min, and was highly stable across a range of temperatures and pH. JSSK01 infected 59.3% of the MDR strains tested, and its depolymerase (ORF40) specifically degraded the K1 capsule in these bacteria. In a zebrafish model, JSSK01 treatment after infection significantly improved survival, with survival in the treated group reaching 100%, while that in the untreated group dropped to 10% after three days. The functional activity of depolymerase was validated using zone inhibition and agglutination tests. These results indicate that JSSK01 and its substrate-specific depolymerase have promising therapeutic and diagnostic applications against K1-encapsulated MDR E. coli infections.

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TCMF112186A-01 Buddhist Tzu Chi University

Keywords: Escherichia coli; K1 capsule; depolymerase; endo-N-acetylneuraminidase; multidrug resistance; phage therapy

0 (capsular polysaccharide K1)
0 (Antigens, Bacterial)
0 (Polysaccharides, Bacterial)

Date Created: 20241217 Date Completed: 20241217 Latest Revision: 20250104

20250104

PMC11641727

10.3390/ijms252312497

39684210