Left atrial single-cell transcriptomics reveals amphiregulin as a surrogate marker for atrial fibrillation.

Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE

Original Publication: London, United Kingdom : Nature Publishing Group UK, [2018]-

Amphiregulin*/metabolism ; Amphiregulin*/genetics ; Atrial Fibrillation*/genetics ; Atrial Fibrillation*/metabolism ; Single-Cell Analysis* ; Biomarkers*/blood ; Biomarkers*/metabolism ; Heart Atria*/metabolism ; Transcriptome*; Humans ; Male ; Female ; Macrophages/metabolism ; Monocytes/metabolism ; Middle Aged ; Gene Expression Profiling ; Aged

Atrial fibrillation (AF) is strongly associated with strokes, heart failure, and increased mortality. This study aims to identify the monocyte-macrophage heterogeneity and interactions of these cells with non-immune cells, and to identify functional biomarkers in patients with AF. Therefore, we assess the single cell landscape of left atria (LA), using a combination of single cell and nucleus RNA-seq. Myeloid cells in LA tissue are categorized into five macrophage clusters, three monocyte clusters, and others. Cell-Chat analysis revealed that monocytes and IL1B ⁺ macrophages send epidermal growth factor (EGF) signals to fibroblasts. Amphiregulin (AREG) is the most upregulated gene in monocytes and IL1B ⁺ macrophages in the AF group, compared with healthy controls from other groups. Serum AREG levels are higher in patients with persistent AF. These data suggested that EGF signaling pathway could be a therapeutic target for AF and serum AREG levels provide an effective biomarker for predicting persistent AF.
Competing Interests: Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)

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22K16105 MEXT | Japan Society for the Promotion of Science (JSPS); 18H05429 MEXT | Japan Society for the Promotion of Science (JSPS); 21H04812 MEXT | Japan Society for the Promotion of Science (JSPS); 21H05254 MEXT | Japan Society for the Promotion of Science (JSPS); 19H03653 MEXT | Japan Society for the Promotion of Science (JSPS); 20K21603 MEXT | Japan Society for the Promotion of Science (JSPS); 20H03676 MEXT | Japan Society for the Promotion of Science (JSPS); 22K08206 MEXT | Japan Society for the Promotion of Science (JSPS); JP21gm0910012 Japan Agency for Medical Research and Development (AMED); JP21wm0425001 Japan Agency for Medical Research and Development (AMED); 18069370 Japan Agency for Medical Research and Development (AMED)

0 (Amphiregulin)
0 (Biomarkers)
0 (AREG protein, human)

Date Created: 20241202 Date Completed: 20241202 Latest Revision: 20241205

20241209

PMC11612213

10.1038/s42003-024-07308-w

39622943