Tripartite interactions of PKA catalytic subunit and C-terminal domains of cardiac Ca ²⁺ channel may modulate its β-adrenergic regulation.

Publisher: BioMed Central Country of Publication: England NLM ID: 101190720 Publication Model: Electronic Cited Medium: Internet ISSN: 1741-7007 (Electronic) Linking ISSN: 17417007 NLM ISO Abbreviation: BMC Biol Subsets: MEDLINE

Original Publication: [London] : BioMed Central, c2003-

Calcium Channels, L-Type*/metabolism ; Calcium Channels, L-Type*/genetics ; Calcium Channels, L-Type*/chemistry ; Cyclic AMP-Dependent Protein Kinases*/metabolism ; Receptors, Adrenergic, beta*/metabolism; Animals ; Catalytic Domain ; Myocytes, Cardiac/metabolism ; Mice ; Protein Domains ; Humans

Competing Interests: Declarations. Ethics approval and consent to participate: Experiments were approved by Tel Aviv University Institutional Animal Care and Use Committee (IACUC permit # 01–20-083). Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests.
Background: The β-adrenergic augmentation of cardiac contraction, by increasing the conductivity of L-type voltage-gated Ca V 1.2 channels, is of great physiological and pathophysiological importance. Stimulation of β-adrenergic receptors (βAR) activates protein kinase A (PKA) through separation of regulatory (PKAR) from catalytic (PKAC) subunits. Free PKAC phosphorylates the inhibitory protein Rad, leading to increased Ca ²⁺ influx. In cardiomyocytes, the core subunit of Ca V 1.2, Ca V 1.2α 1 , exists in two forms: full-length or truncated (lacking the distal C-terminus (dCT)). Signaling efficiency is believed to emanate from protein interactions within multimolecular complexes, such as anchoring PKA (via PKAR) to Ca V 1.2α 1 by A-kinase anchoring proteins (AKAPs). However, AKAPs are inessential for βAR regulation of Ca V 1.2 in heterologous models, and their role in cardiomyocytes also remains unclear.
Results: We show that PKAC interacts with Ca V 1.2α 1 in heart and a heterologous model, independently of Rad, PKAR, or AKAPs. Studies with peptide array assays and purified recombinant proteins demonstrate direct binding of PKAC to two domains in Ca V 1.2α 1 -CT: the proximal and distal C-terminal regulatory domains (PCRD and DCRD), which also interact with each other. Data indicate both partial competition and possible simultaneous interaction of PCRD and DCRD with PKAC. The βAR regulation of Ca V 1.2α 1 lacking dCT (which harbors DCRD) was preserved, but subtly altered, in a heterologous model, the Xenopus oocyte.
Conclusions: We discover direct interactions between PKAC and two domains in Ca V 1.2α 1 . We propose that these tripartite interactions, if present in vivo, may participate in organizing the multimolecular signaling complex and fine-tuning the βAR effect in cardiomyocytes.
(© 2024. The Author(s).)

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Keywords: Adrenergic regulation; Calcium channel; Cardiac; Heterologous expression; Protein kinase A (PKA); Protein‐protein interaction

0 (Calcium Channels, L-Type)
EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
0 (Receptors, Adrenergic, beta)

Date Created: 20241128 Date Completed: 20241129 Latest Revision: 20241201

20250114

PMC11603854

10.1186/s12915-024-02076-9

39609812