Novel polyomavirus in the endangered garden dormouse Eliomys quercinus.

Publisher: BioMed Central Country of Publication: England NLM ID: 101231645 Publication Model: Electronic Cited Medium: Internet ISSN: 1743-422X (Electronic) Linking ISSN: 1743422X NLM ISO Abbreviation: Virol J Subsets: MEDLINE

Original Publication: [London] : BioMed Central, 2004-

Polyomavirus*/genetics ; Polyomavirus*/isolation & purification ; Polyomavirus*/classification ; Phylogeny*; Animals ; Germany/epidemiology ; Seroepidemiologic Studies ; DNA, Viral/genetics ; Genome, Viral ; Polyomavirus Infections/virology ; Polyomavirus Infections/epidemiology ; Sequence Analysis, DNA ; Kidney/virology ; Polymerase Chain Reaction ; Murinae/virology ; Enzyme-Linked Immunosorbent Assay

Background: The garden dormouse (Eliomys quercinus) has experienced a significant population decline across Europe in recent decades. While habitat loss and climate change are often cited as primary factors, pathogen exposure, either to novel or to previously known, may play a role in such a decline. This study aimed to investigate the presence of polyomaviruses in garden dormice, given that these viruses are highly prevalent and can cause disease, particularly in immunocompromised individuals.
Methods: The carcasses of garden dormice (n = 89) were collected throughout Germany. Kidney samples were tested for the presence of polyomavirus DNA using nested degenerate and specific diagnostic PCRs. Seroprevalence was assessed from chest cavity fluid samples through an enzyme-linked immunosorbent assay using polyomavirus VP1 virus-like particles produced in yeast.
Results: A new polyomavirus, related to chimpanzee (Pan troglodytes) polyomaviruses 4 and 5 and human Merkel cell polyomavirus, was identified in the garden dormouse. Two 5,380 bp-length complete viral genomes were sequenced from dormice kidney samples (sequences PQ246041 and PQ246042). Genes encoding the putative structural proteins VP1, VP2, and VP3, as well as the Large, Middle, and small T antigens, containing conserved functional domains were identified. Polyomavirus DNA was detected in 2 of 74 dormice (2.7%, 95% confidence interval: 0-6.4%) through PCR, while 12 of 69 animals (17.4%, 95% confidence interval: 8.4-26.3%) tested positive for polyomavirus-specific antibodies.
Conclusions: In conclusion, here we describe a novel polyomavirus in the garden dormouse with molecular and serological detection. Pairwise sequence comparison and phylogenetic analysis suggest that this novel virus may represent a novel species within the genus Alphapolyomavirus. Future work should examine if this virus is garden dormouse-specific and whether it is associated with disease in dormice.
Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)

Bioinformatics. 2006 Jul 1;22(13):1658-9. (PMID: 16731699)
Virology. 2021 Nov;563:58-63. (PMID: 34425496)
J Med Virol. 2023 Oct;95(10):e29197. (PMID: 37881064)
Front Microbiol. 2022 Feb 18;13:834368. (PMID: 35250950)
PLoS One. 2015 Oct 16;10(10):e0140916. (PMID: 26474048)
mSphere. 2016 Dec 21;1(6):. (PMID: 28028546)
J Natl Cancer Inst. 1968 Apr;40(4):867-73. (PMID: 5646499)
Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2403133121. (PMID: 39141346)
Vet Pathol. 2023 Mar;60(2):267-275. (PMID: 36537739)
Viruses. 2023 Nov 09;15(11):. (PMID: 38005912)
Acta Unio Int Contra Cancrum. 1963;19:362-7. (PMID: 14034720)
Mol Cell. 2016 Aug 18;63(4):686-695. (PMID: 27453045)
PLoS One. 2018 Oct 23;13(10):e0206273. (PMID: 30352098)
Microbiol Resour Announc. 2019 Jul 18;8(29):. (PMID: 31320414)
Nucleic Acids Res. 2002 Jul 15;30(14):3059-66. (PMID: 12136088)
Annu Rev Virol. 2016 Sep 29;3(1):517-532. (PMID: 27501263)
Nature. 1994 May 19;369(6477):207-12. (PMID: 7910375)
Bioinformatics. 2020 Apr 1;36(7):2280-2281. (PMID: 31755898)
Arch Virol. 2018 Oct;163(10):2913-2915. (PMID: 29931397)
mBio. 2018 Apr 17;9(2):. (PMID: 29666290)
Cell Discov. 2017 Aug 08;3:17027. (PMID: 28884018)
Infect Genet Evol. 2024 Sep;123:105620. (PMID: 38876268)
Front Microbiol. 2018 Mar 08;9:398. (PMID: 29568287)
Arch Virol. 2013 Nov;158(11):2255-65. (PMID: 23719671)
Mol Biol Evol. 2022 Aug 06;:. (PMID: 35932227)
Toxicol Pathol. 2017 Jul;45(5):593-603. (PMID: 28782456)
Eur J Clin Microbiol Infect Dis. 2019 Jan;38(1):135-139. (PMID: 30338464)
J Virol. 1991 Jun;65(6):3301-8. (PMID: 1709702)
Appl Environ Microbiol. 2014 Jun;80(12):3615-21. (PMID: 24705325)
J Gen Virol. 2011 Apr;92(Pt 4):789-95. (PMID: 21177925)
Annu Rev Microbiol. 2012;66:213-36. (PMID: 22994493)
Viruses. 2021 May 13;13(5):. (PMID: 34068409)
Mol Cell Biol. 1992 Nov;12(11):4883-95. (PMID: 1328866)
Nat Methods. 2021 Apr;18(4):366-368. (PMID: 33828273)
Bioinformatics. 2019 Nov 1;35(21):4453-4455. (PMID: 31070718)
Int J Mol Sci. 2023 Mar 03;24(5):. (PMID: 36902338)
Virol Sin. 2022 Aug;37(4):491-502. (PMID: 35680114)
Viruses. 2019 Oct 10;11(10):. (PMID: 31658738)
Arch Virol. 2018 Nov;163(11):3203-3206. (PMID: 30097743)
Infect Genet Evol. 2014 Aug;26:283-94. (PMID: 24933462)
PLoS Pathog. 2016 Apr 19;12(4):e1005574. (PMID: 27093155)
J Virol. 2011 Apr;85(7):3046-54. (PMID: 21228238)
Transfusion. 2019 Dec;59(12):3689-3697. (PMID: 31633816)
Nucleic Acids Res. 2024 Jul 5;52(W1):W78-W82. (PMID: 38613393)
Am J Epidemiol. 2016 Jan 1;183(1):61-9. (PMID: 26667254)
Genome Res. 2024 Nov 20;34(11):2094-2107. (PMID: 39542649)
Gene. 1994 Nov 18;149(2):311-4. (PMID: 7959008)
Cell Cycle. 2009 Feb 1;8(3):1-7. (PMID: 19182532)
J Virol. 1992 Jul;66(7):3979-85. (PMID: 1318392)
Proc Soc Exp Biol Med. 1953 Jan;82(1):133-7. (PMID: 13037823)
Genome Announc. 2015 Sep 03;3(5):. (PMID: 26337891)
Front Microbiol. 2021 Oct 18;12:740947. (PMID: 34733257)
BMC Biotechnol. 2015 Aug 04;15:68. (PMID: 26239840)
J Virol. 2005 Mar;79(6):3883-7. (PMID: 15731285)
Nat Rev Nephrol. 2012 Apr 17;8(7):390-402. (PMID: 22508181)
Arch Virol. 2020 Oct;165(10):2291-2299. (PMID: 32754877)
Nat Methods. 2024 Jun;21(6):994-1002. (PMID: 38755321)
J Gen Virol. 2017 Dec;98(12):3060-3067. (PMID: 29095685)
Proc Soc Exp Biol Med. 1953 Jun;83(2):414-21. (PMID: 13064287)
Virology. 2009 Feb 20;384(2):352-9. (PMID: 19101707)
Arch Virol. 2023 Sep 15;168(10):253. (PMID: 37715108)
Virol J. 2020 Mar 27;17(1):42. (PMID: 32220234)
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3110-4. (PMID: 7536925)
Intervirology. 2002;45(4-6):308-17. (PMID: 12602348)
Viruses. 2023 Jul 13;15(7):. (PMID: 37515232)
Viruses. 2022 Sep 19;14(9):. (PMID: 36146885)
Genome Biol. 2019 Nov 5;20(1):232. (PMID: 31690338)
Trends Microbiol. 2010 May;18(5):215-23. (PMID: 20176487)
Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2316467120. (PMID: 38079542)
BMC Ecol. 2017 Feb 28;17(1):9. (PMID: 28245831)

KD-20185 European Union under the agreement with the Research Council of Lithuania (LMTLT); KD-20185 European Union under the agreement with the Research Council of Lithuania (LMTLT); KD-20185 European Union under the agreement with the Research Council of Lithuania (LMTLT)

Keywords: Complete genome; Garden dormouse; Infection; Phylogeny; Polyomavirus; Seroprevalence; Virus-like particles

0 (DNA, Viral)

Date Created: 20241128 Date Completed: 20241128 Latest Revision: 20241130

20241202

PMC11603729

10.1186/s12985-024-02581-x

39605065