Participation of Semaphorin Family and Plexins in the Clinical Course of Patients with Inflammatory Bowel Disease.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Semaphorins*/metabolism ; Semaphorins*/genetics ; Inflammatory Bowel Diseases*/metabolism ; Inflammatory Bowel Diseases*/genetics ; Inflammatory Bowel Diseases*/pathology ; Receptors, Cell Surface*/metabolism ; Receptors, Cell Surface*/genetics ; Nerve Tissue Proteins*/metabolism ; Nerve Tissue Proteins*/genetics; Humans ; Female ; Male ; Adult ; Middle Aged ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/pathology ; Colitis, Ulcerative/metabolism ; Colitis, Ulcerative/genetics ; Colitis, Ulcerative/pathology ; Cross-Sectional Studies ; Case-Control Studies ; Antigens, CD/metabolism ; Antigens, CD/genetics ; Cell Adhesion Molecules

Semaphorins are an immunoregulatory protein family. Plexins bind semaphorins (SEMAs) and can form receptor complexes that give them chemotactic capacity. The role and expression profile of semaphorins and plexins in inflammatory bowel disease (IBD) is currently unknown.
Aim: Characterize the semaphorins and plexins gene and protein expression in intestinal tissue from IBD patients and correlate them with the clinical phenotype.
Material and Methods: This comparative and cross-sectional study enrolled 54 diagnosed IBD patients and 20 controls. Gene and protein expression of semaphorins and plexins were determined by RT-PCR and IHQ for the co-localization with neutrophils (myeloperoxidase, MPO) or CD123 plasmacytoid dendritic cells in intestinal tissue from IBD patients.
Results: Colonic mucosa from active and remission ulcerative colitis (UC) had a significantly lower SEMA4D and PLXNA1 , but higher PLXNB1 gene expression than the control group. The only significant difference between active UC and remission was observed in the higher gene expression of SEMA6D in remission. It was associated with histological remission ( p = 0.01, OR = 15, 95% CI: 1.39-16.1). The low expression of PLXNA1 was associated with mild intermittent activity with two relapses per year ( p = 0.003, OR = 0.05, CI = 0.006-0.51). Higher SEMA4D+ positive cells were detected in the submucosa, while PLXNC1+/MPO+ in the mucosal and submucosa of active UC patients compared with controls.
Conclusions: The increased expression of the semaphorin and plexin family in IBD patients suggests their immunoregulatory function and is associated with remission and clinical phenotype in patients with UC.

Immunol Res. 2024 Apr;72(2):284-292. (PMID: 37968544)
Mucosal Immunol. 2014 Jan;7(1):134-42. (PMID: 23695512)
Nat Rev Rheumatol. 2018 Jan;14(1):19-31. (PMID: 29213125)
Cell Rep. 2019 Mar 26;26(13):3698-3708.e5. (PMID: 30917322)
Inflamm Bowel Dis. 2015 Sep;21(9):2188-93. (PMID: 26111210)
Scand J Immunol. 2021 Apr;93(4):e13004. (PMID: 33247598)
Nat Rev Immunol. 2008 Jun;8(6):458-66. (PMID: 18500230)
Biochemistry (Mosc). 2019 Sep;84(9):1021-1027. (PMID: 31693461)
PLoS One. 2015 May 15;10(5):e0125860. (PMID: 25978359)
Rev Gastroenterol Mex (Engl Ed). 2019 Jul - Sep;84(3):317-325. (PMID: 30679027)
Cell. 1993 Dec 31;75(7):1389-99. (PMID: 8269517)
N Engl J Med. 2002 Aug 8;347(6):417-29. (PMID: 12167685)
Immunol Invest. 2020 Feb;49(1-2):69-80. (PMID: 31412748)
Immunol Res. 2021 Oct;69(5):429-435. (PMID: 34327631)
Nat Immunol. 2018 Jun;19(6):561-570. (PMID: 29777213)
Oncol Lett. 2016 Nov;12(5):3967-3974. (PMID: 27895757)
Immunobiology. 2021 May;226(3):152095. (PMID: 34000572)
Front Immunol. 2020 Mar 05;11:346. (PMID: 32210960)
EMBO J. 2020 Jul 1;39(13):e103325. (PMID: 32510170)
Nat Immunol. 2008 Jan;9(1):17-23. (PMID: 18087252)
Immunity. 2006 May;24(5):591-600. (PMID: 16713976)

Keywords: IBD; plexins; semaphorins

0 (Semaphorins)
0 (Receptors, Cell Surface)
0 (Nerve Tissue Proteins)
0 (PLXNB1 protein, human)
0 (PLXNA1 protein, human)
0 (plexin)
0 (Antigens, CD)
0 (Cell Adhesion Molecules)

Date Created: 20241127 Date Completed: 20241127 Latest Revision: 20241130

20241202

PMC11595178

10.3390/ijms252212442

39596507