Molecular Insights into the Reproductive Patterns and Genetic Structure of Wheat Stripe Rust in Ili, Xinjiang.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Triticum*/microbiology ; Triticum*/genetics ; Plant Diseases*/microbiology ; Plant Diseases*/genetics ; Genetic Variation* ; Puccinia*/pathogenicity ; Puccinia*/genetics ; Microsatellite Repeats*/genetics; China ; Basidiomycota/genetics ; Gene Flow ; Phylogeny

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is a globally significant fungal disease that seriously threatens wheat yield, particularly in China. This study investigates the genetic structure and reproductive patterns of Pst populations in Ili, Xinjiang, using 12 pairs of Simple Sequence Repeat (SSR) molecular markers. Analyses of 79 Pst isolates from either spring or winter wheat areas in Ili revealed three primary genetic clusters, indicating notable differences between populations associated with spring and winter wheat. The STRUCTURE results, complemented by UPGMA and PCoA analyses, highlight significant genetic diversity within these populations, with evidence of genetic recombination and sexual reproduction in certain areas. Pst populations in Ili exhibit a mixed mode of reproduction, predominantly sexual in Qapqal and Xinyuan D and primarily asexual within the spring wheat populations. The gene flow analysis underscores extensive inter-population communication, which facilitates the spread and adaptation of the pathogen across diverse wheat-growing environments. This study marks the first documentation of sexual reproduction in Pst within Xinjiang, providing new insights into its spread and genetic variation. These findings suggest that sexual reproduction may play a role in the regional adaptation and evolution of Pst , impacting future management strategies for wheat stripe rust in Xinjiang and potentially in broader Central Asian contexts.

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CARS-03-88 Urumqi Integrated Experimental Station of China Agriculture Research System for Wheat; 2023E01015 the Regional Coordinated Innovation Project, Shanghai Cooperation Organization Science and Technology Partnership Program; XJARS-01 the earmarked fund; 2022D01A270 Natural Science Foundation of Xinjiang Uygur Autonomous Region; XJNKYWDZC-2023004-1 Project of Fund for Stable Support to Agricultural Sci-Tech Renovation

Keywords: genetic structure; sexual reproductive; simple sequence repeats; wheat stripe rust

Puccinia striiformis

Date Created: 20241127 Date Completed: 20241127 Latest Revision: 20241130

20241202

PMC11594354

10.3390/ijms252212357

39596420