Hybrid Origin of × Leymotrigia bergrothii (Poaceae) as Revealed by Analysis of the Internal Transcribed Spacer ITS1 and trn L Sequences.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Poaceae*/genetics ; Poaceae*/classification; DNA, Ribosomal Spacer/genetics ; Phylogeny ; Hybridization, Genetic ; Polymorphism, Genetic ; Genome, Plant ; DNA, Plant/genetics

× Leymotrigia bergrothii is a presumed hybrid of Leymus arenarius and Elytrigia repens . This article investigates the hybrid origin and genome composition of this species. These plants are sterile, do not undergo pollination, and do not produce seeds; occasionally, underdeveloped stamens containing abortive pollen grains form in individual spikelets. The karyotype analysis of root meristem cells revealed a diploid chromosome number of 49 in × L. bergrothii , reported here for the first time. Subsequently, we examined the intragenomic polymorphism of the transcribed spacer ITS1 in several species of Elytrigia , Elymus , Leymus , Hordeum , and Psathyrostachys , and compared the ribotype patterns of these species with those of × L. bergrothii. It is shown that the St-ribotype variants found in Elytrigia repens and Elytrigia pseudocaesia , as well as the ribotypes of the La family, which dominate in the genome of Leymus arenarius , correspond to major ribotypes in × L. bergrothii . The ribotypes of the St and La families are present in the nuclear genome of × L. bergrothii in almost equal proportions. A comparison of intron and exon sequences of the trn L gene in the chloroplast DNA of L ey mus arenarius , Elytrigia repens , and × L. bergrothii showed that this region in × L. bergrothii is identical or very close to that of Elytrigia repens , suggesting that Elytrigia repens was the cytoplasmic donor to × L. bergrothii. Thus, our study confirms the hypothesis that this species represents a sterile first-generation hybrid of Leymus arenarius and Elytrigia repens , reproducing vegetatively.

Mol Ecol Resour. 2015 May;15(3):573-86. (PMID: 25187125)
Bioinformatics. 2012 Apr 15;28(8):1166-7. (PMID: 22368248)
Sci Rep. 2022 Oct 7;12(1):16907. (PMID: 36207443)
PLoS One. 2018 Oct 25;13(10):e0206428. (PMID: 30359454)
PeerJ. 2016 Oct 18;4:e2584. (PMID: 27781170)
Mol Phylogenet Evol. 2008 May;47(2):598-611. (PMID: 18372193)
Proc Natl Acad Sci U S A. 1996 May 14;93(10):5090-3. (PMID: 11607681)
BMC Ecol. 2003 Oct 16;3:8. (PMID: 14563214)
Plant Mol Biol. 1991 Nov;17(5):1105-9. (PMID: 1932684)
Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
Plant Mol Biol. 2000 Jun;43(2-3):163-77. (PMID: 10999402)
Plant Mol Biol. 1987 Sep;9(5):509-20. (PMID: 24277137)
Bioinformatics. 2016 Feb 15;32(4):627-8. (PMID: 26515821)
BMC Genet. 2016 Dec 5;17(1):150. (PMID: 27919229)
BMC Genomics. 2012 Dec 23;13:722. (PMID: 23259460)
Mol Biol Evol. 2010 Jun;27(6):1370-90. (PMID: 20106909)
Mol Phylogenet Evol. 2014 Aug;77:296-306. (PMID: 24780748)
Front Plant Sci. 2021 Dec 21;12:797348. (PMID: 34992624)
Genetics. 1992 Oct;132(2):619-33. (PMID: 1385266)
Nat Plants. 2018 May;4(5):258-268. (PMID: 29725103)
Genome. 2003 Feb;46(1):156-63. (PMID: 12669808)
BMC Evol Biol. 2017 Jun 16;17(1):141. (PMID: 28622761)
Plants (Basel). 2024 Mar 22;13(7):. (PMID: 38611448)
Plant J. 2020 Aug;103(5):1810-1825. (PMID: 32506573)
Plant J. 2023 May;114(4):743-766. (PMID: 36775995)
Plants (Basel). 2022 Dec 15;11(24):. (PMID: 36559646)
Heredity (Edinb). 2023 Sep;131(3):179-188. (PMID: 37402824)
Mol Phylogenet Evol. 2017 Sep;114:175-188. (PMID: 28533082)
Am J Bot. 2001 Apr;88(4):553-9. (PMID: 11302839)
Mol Ecol. 2000 Oct;9(10):1657-9. (PMID: 11050560)
Front Zool. 2009 Aug 20;6:16. (PMID: 19695081)
Plant J. 2023 Sep;115(5):1298-1315. (PMID: 37246611)
Sci Rep. 2022 Dec 14;12(1):21610. (PMID: 36517537)

Agreement No. 075-15-2021-1056 The Ministry of Education and Science of the Russian Federation

Keywords: Elymus; Elytrigia; Leymus; Triticeae; hybridization; molecular phylogeny

0 (DNA, Ribosomal Spacer)
0 (DNA, Plant)

Date Created: 20241127 Date Completed: 20241127 Latest Revision: 20241130

20241202

PMC11594234

10.3390/ijms252211966

39596035