Correlation Between mRNA Expression of Activated Eosinophils and Air Pollutant Exposure in Patients With Asthma.

Publisher: John Wiley and Sons Ltd Country of Publication: England NLM ID: 101635460 Publication Model: Print Cited Medium: Internet ISSN: 2050-4527 (Electronic) Linking ISSN: 20504527 NLM ISO Abbreviation: Immun Inflamm Dis Subsets: MEDLINE

Original Publication: [Oxford] : John Wiley and Sons Ltd, [2013]-

Eosinophils*/immunology ; Eosinophils*/metabolism ; Asthma*/genetics ; Asthma*/immunology ; Asthma*/metabolism ; RNA, Messenger*/genetics ; Air Pollutants*/adverse effects; Humans ; Male ; Female ; Middle Aged ; Adult ; Air Pollution/adverse effects ; Transforming Growth Factor beta1/metabolism ; Transforming Growth Factor beta1/genetics ; Interleukin-5/genetics ; Environmental Exposure/adverse effects

Background: Eosinophil activation is associated with asthma. Whether air pollution affects the activation of blood eosinophils in patients with asthma remains unknown. In this study, we investigated the correlation between transcriptional activity in eosinophils and air pollutant exposure in patients receiving different levels of Global Initiative for Asthma (GINA) treatment.
Methods: We evaluated the expression levels of activation- and function-related genes in eosinophils from patients with GINA 4 or 5 (n = 20), those with GINA 3 (n = 12), and normal individuals (n = 7); the eosinophils were activated with interleukin (IL)-5 or IL-17. A land use regression model was used to estimate air pollutant exposure. The correlations between mRNA expression, lung function, and air pollutant exposure were investigated.
Results: The expression levels of TGFB1, IL7R, and TLR3 were significantly higher for patients with GINA 4 or 5 than for those with GINA 3 or normal individuals. The expression of certain genes, particularly in IL-17-activated eosinophils, was correlated with lung function decline in patients with GINA 4 or 5. For patients with GINA 4 or 5, NO 2 exposure was correlated with upregulated TGFB1 expression in IL-5-activated eosinophils. For patients with GINA 3, O 3 exposure was correlated with upregulated CCR5, IL5RA, IL7R, and TGFB1 expression in IL-17-activated eosinophils and upregulated IL7R expression in IL-5-activated eosinophils.
Conclusion: Patients with GINA 4 or 5 may exhibit elevated transcriptional activity in eosinophils; this elevation is correlated with lung function decline. Air pollution may affect eosinophil mRNA expression in patients with asthma.
(© 2024 The Author(s). Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.)

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This study was supported by Chang Gung Memorial Hospital (grant number: CMRPG3L1261).

Keywords: air pollution; asthma; eosinophil; mRNA

0 (RNA, Messenger)
0 (Air Pollutants)
0 (Transforming Growth Factor beta1)
0 (Interleukin-5)
0 (TGFB1 protein, human)

Date Created: 20241122 Date Completed: 20241122 Latest Revision: 20241124

20241126

PMC11582923

10.1002/iid3.70065

39575691