Comparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signalling.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Cryptochromes*/metabolism ; Cryptochromes*/genetics ; Signal Transduction* ; Protein Binding*; Animals ; Retinal Cone Photoreceptor Cells/metabolism ; Vitamin A/metabolism ; Surface Plasmon Resonance ; Recombinant Proteins/metabolism ; Recombinant Proteins/genetics ; GTP-Binding Protein alpha Subunits/metabolism ; GTP-Binding Protein alpha Subunits/genetics ; Fluorescence Resonance Energy Transfer

Vision and magnetoreception in navigating songbirds are strongly connected as recent findings link a light dependent radical-pair mechanism in cryptochrome proteins to signalling pathways in cone photoreceptor cells. A previous yeast-two-hybrid screening approach identified six putative candidate proteins showing binding to cryptochrome type 4a. So far, only the interaction of the cone specific G-protein transducin α-subunit was investigated in more detail. In the present study, we compare the binding features of the G-protein α-subunit with those of another candidate from the yeast-two-hybrid screen, cellular retinol binding protein. Purified recombinant European robin retinol binding protein bound retinol with high affinity, displaying an EC 50 of less than 5 nM, thereby demonstrating its functional state. We applied surface plasmon resonance and a Förster resonance transfer analysis to test for interactions between retinol binding protein and cryptochrome 4a. In the absence of retinol, we observed no robust binding events, which contrasts the strong interaction we observed between cryptochrome 4a and the G-protein α-subunit. We conclude that retinol binding protein is unlikely to be involved in the primary magnetosensory signalling cascade.
Competing Interests: Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)

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Keywords: Cryptochrome; G protein; Magnetoreception; Protein–protein interaction; Retinol binding protein

0 (Cryptochromes)
11103-57-4 (Vitamin A)
0 (Recombinant Proteins)
0 (GTP-Binding Protein alpha Subunits)

Date Created: 20241116 Date Completed: 20241117 Latest Revision: 20241122

20241122

PMC11569197

10.1038/s41598-024-79699-z

39550406