The circulatory levels and bone expression of MIR21, 34a, 155 and their target genes in a section of Egyptian Population.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

MicroRNAs*/genetics ; MicroRNAs*/blood ; Bone and Bones*/metabolism; Humans ; Female ; Male ; Adult ; Middle Aged ; Egypt ; Alkaline Phosphatase/blood ; Alkaline Phosphatase/genetics ; Alkaline Phosphatase/metabolism ; Prospective Studies ; Osteocalcin/blood ; Osteocalcin/genetics ; Biomarkers/blood ; Bone Remodeling/genetics ; Gene Expression Regulation

Bone tissue is constantly regenerated and repaired through a finely balanced process known as bone remodeling. Many miRNAs act as regulators of the signaling pathways involved in bone metabolic processes to maintain tissue homeostasis. This study aimed to assess the circulating levels of MIR21, MIR34a, and MIR155 in human serum and their bone expression, and the expression of bone turnover-related genes which can reflect the bone quality. This prospective study was conducted on 60 patients (30 males and 30 females) indicated for surgical interventions for neural decompression +/- fixation. Relative quantification of expression of MIR21, miR34a, and MIR155 and bone related genes was assayed using PCR. The serum levels of osteocalcin and Serum Bone Alkaline Phosphatase (sBAP) were assayed using a human ELISA kit. The main finding of the present work was the strong positive association between the circulating levels of only miR21 and MIR155 and their bone expression in the population under study and with bone markers and target genes Also, a positive association was found between both bone expression and circulating MIR21 levels with age and sBAP. These results suggest that the circulating levels of these microRNAs as early markers for the predication of bone quality.
Competing Interests: Declarations Competing interests The authors declare no competing interests. Ethics approval This study was performed in line with the Declaration of Helsinki, and approved by Ethics Committee of Medical Research Institute, Alexandria University, Egypt. (Approval serial number : E/C, S/N, R18/2020 in October 2020).” for studies involving humans. Consent to participate Informed consent was obtained from all individual participants included in the study. Patient consent for publication Written informed consent has been obtained from the patient to publish this paper .
(© 2024. The Author(s).)

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Keywords: Bone metabolism; Bone quality; Egyptian population; MicroRNA; Osteocalcin

0 (MicroRNAs)
EC 3.1.3.1 (Alkaline Phosphatase)
0 (MIRN21 microRNA, human)
104982-03-8 (Osteocalcin)
0 (Biomarkers)
0 (MIRN155 microRNA, human)

Date Created: 20241113 Date Completed: 20241113 Latest Revision: 20241116

20241116

PMC11561067

10.1038/s41598-024-77643-9

39537688