GelMA hydrogels reinforced by PCL@GelMA nanofibers and bioactive glass induce bone regeneration in critical size cranial defects.

Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE

Original Publication: London : BioMed Central, 2003-

Bone Regeneration*/drug effects ; Hydrogels*/chemistry ; Nanofibers*/chemistry ; Mesenchymal Stem Cells*/drug effects ; Mesenchymal Stem Cells*/cytology ; Mesenchymal Stem Cells*/metabolism ; Osteogenesis*/drug effects ; Skull*/drug effects ; Skull*/injuries ; Glass*/chemistry; Animals ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Polyesters/chemistry ; Male ; Tissue Scaffolds/chemistry ; Rats, Sprague-Dawley ; Rats ; Tissue Engineering/methods

Background: The process of bone healing is complex and involves the participation of osteogenic stem cells, extracellular matrix, and angiogenesis. The advancement of bone regeneration materials provides a promising opportunity to tackle bone defects. This study introduces a composite hydrogel that can be injected and cured using UV light.
Results: Hydrogels comprise bioactive glass (BG) and PCL@GelMA coaxial nanofibers. The addition of BG and PCL@GelMA coaxial nanofibers improves the hydrogel's mechanical capabilities (353.22 ± 36.13 kPa) and stability while decreasing its swelling (258.78 ± 17.56%) and hydration (72.07 ± 1.44%) characteristics. This hydrogel composite demonstrates exceptional biocompatibility and angiogenesis, enhances osteogenic development in bone marrow mesenchymal stem cells (BMSCs), and dramatically increases the expression of critical osteogenic markers such as ALP, RUNX2, and OPN. The composite hydrogel significantly improves bone regeneration (25.08 ± 1.08%) in non-healing calvaria defects and promotes the increased expression of both osteogenic marker (OPN) and angiogenic marker (CD31) in vivo. The expression of OPN and CD31 in the composite hydrogel was up to 5 and 1.87 times higher than that of the control group at 12 weeks.
Conclusion: We successfully prepared a novel injectable composite hydrogel, and the design of the composite hydrogels shows significant potential for enhancing biocompatibility, angiogenesis, and improving osteogenic and angiogenic marker expression, and has a beneficial effect on producing an optimal microenvironment that promotes bone repair.
Competing Interests: Declarations Ethics approval and consent to participate All animal experiment studies were approved by the animal ethics committee of Qingdao University (No.20210901SD5020231101128, Shandong, China) and conducted in accordance with the National Research Council’s Guide for the Care and Use of Laboratory Animals. Consent for publication Not applicable. Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)

Adv Healthc Mater. 2023 Apr;12(11):e2202163. (PMID: 36637340)
Bone Res. 2017 May 30;5:17014. (PMID: 28584674)
Front Bioeng Biotechnol. 2019 Jul 05;7:161. (PMID: 31334228)
J Orthop Translat. 2022 Nov 03;37:152-162. (PMID: 36380884)
Bone. 2015 Dec;81:112-121. (PMID: 26163110)
Materials (Basel). 2019 Jun 28;12(13):. (PMID: 31261615)
Adv Healthc Mater. 2022 Jul;11(13):e2200298. (PMID: 35388979)
Adv Sci (Weinh). 2021 Oct;8(20):e2100894. (PMID: 34396718)
J Biomed Mater Res A. 2021 May;109(5):590-599. (PMID: 32588539)
J Biomed Mater Res A. 2016 Apr;104(4):879-88. (PMID: 26650507)
J Biomed Mater Res. 1971 Jan;5(1):25-51. (PMID: 4995062)
Sci Rep. 2016 Aug 09;6:31036. (PMID: 27503340)
Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:1137-46. (PMID: 27612811)
Biomed Mater. 2021 Apr 01;16(4):042007. (PMID: 33793421)
Mater Today Bio. 2024 May 22;26:101099. (PMID: 38840797)
Sci Rep. 2019 May 3;9(1):6863. (PMID: 31053756)
Tissue Eng Part B Rev. 2020 Apr;26(2):164-180. (PMID: 31910095)
Bioengineering (Basel). 2019 Aug 28;6(3):. (PMID: 31466260)
J Mater Chem B. 2014 Jun 7;2(21):3161-3184. (PMID: 24999429)
Nat Protoc. 2016 Apr;11(4):727-46. (PMID: 26985572)
ACS Appl Mater Interfaces. 2023 May 3;15(17):21476-21495. (PMID: 37073785)
Nanoscale. 2024 May 23;16(20):9861-9874. (PMID: 38712977)
J Biomed Mater Res B Appl Biomater. 2023 Jan;111(1):173-183. (PMID: 35938837)
J Colloid Interface Sci. 2021 Aug 15;596:312-323. (PMID: 33839356)
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:233-241. (PMID: 28482522)
Adv Drug Deliv Rev. 2018 Mar 1;127:167-184. (PMID: 29567395)
Biomolecules. 2021 Mar 17;11(3):. (PMID: 33802745)
J Funct Biomater. 2022 Dec 31;14(1):. (PMID: 36662070)
Mater Sci Eng C Mater Biol Appl. 2015 Nov 1;56:473-80. (PMID: 26249617)
Mater Sci Eng C Mater Biol Appl. 2021 May;124:112050. (PMID: 33947544)
ACS Appl Mater Interfaces. 2017 Nov 29;9(47):41168-41180. (PMID: 29144723)
Biochem Biophys Res Commun. 2010 Feb 26;393(1):156-61. (PMID: 20117086)
Biomater Adv. 2023 Nov;154:213616. (PMID: 37708668)
J Tissue Eng Regen Med. 2022 Feb;16(2):200-206. (PMID: 34844287)
J Mech Behav Biomed Mater. 2017 Apr;68:8-15. (PMID: 28135640)
J Funct Biomater. 2022 Jun 05;13(2):. (PMID: 35735929)
Bioact Mater. 2021 Mar 21;6(10):3485-3495. (PMID: 33817422)
Sci Rep. 2023 Apr 25;13(1):6775. (PMID: 37185618)
Biomaterials. 2022 Jun;285:121538. (PMID: 35504180)
J Biomed Mater Res. 2000 Jul;51(1):37-46. (PMID: 10813743)
J Biomed Mater Res A. 2008 Jul;86(1):31-40. (PMID: 17941016)
Mater Sci Eng C Mater Biol Appl. 2018 Aug 1;89:119-127. (PMID: 29752080)
Int J Nanomedicine. 2021 Aug 31;16:6049-6065. (PMID: 34511905)
Tissue Eng Part B Rev. 2010 Apr;16(2):199-207. (PMID: 19831556)
J Appl Biomater. 1992 Summer;3(2):123-9. (PMID: 10147709)
J Biomed Mater Res B Appl Biomater. 2022 Apr;110(4):910-922. (PMID: 34936202)

ZR2021QC063 Natural Science Foundation of Shandong Province; ZR2021YQ17 Natural Science Foundation of Shandong Province; 2022-2024 Qingdao Key Health Discipline Development Fund; 22-3-7-lczx-7-nsh Qingdao Clinical Research Center for Oral Diseases; 2024-2026 Shandong Provincial Key Medical and Health Discipline of Oral Medicine; 2021SFGC0502 Key Technology Research and Development Program of Shandong Province; No. tsqn202211125 Taishan Scholars Project of Shandong Province; 32171322 National Natural Science Foundation of China; No. YESS20200097 Young Elite Scientists Sponsorship Program by CAST

Keywords: Bioactive glass; Bone regeneration; GelMA; Nanofiber; Organic-inorganic composite

0 (Hydrogels)
0 (Biocompatible Materials)
0 (Polyesters)
24980-41-4 (polycaprolactone)

Date Created: 20241111 Date Completed: 20241112 Latest Revision: 20241116

20250114

PMC11552337

10.1186/s12951-024-02980-w

39529025