Genome Analysis of BnCNGC Gene Family and Function Exploration of BnCNGC57 in Brassica napus L.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Brassica napus*/genetics ; Brassica napus*/metabolism ; Gene Expression Regulation, Plant* ; Cyclic Nucleotide-Gated Cation Channels*/genetics ; Cyclic Nucleotide-Gated Cation Channels*/metabolism ; Phylogeny* ; Multigene Family* ; Plant Proteins*/genetics ; Plant Proteins*/metabolism; Genome, Plant ; Seeds/genetics ; Seeds/growth & development ; Seeds/metabolism ; Stress, Physiological/genetics ; Arabidopsis/genetics ; Arabidopsis/metabolism

The cyclic nucleotide-gated ion channel (CNGC), as a non-selective cation channel, plays a pivotal role in plant growth and stress response. A systematic analysis and identification of the BnCNGC gene family in Brassica napus is crucial for uncovering its biological functions and potential applications in plant science. In this study, we identified 61 BnCNGC members in the B. napus genome, which are phylogenetically similar to Arabidopsis and can be classified into Groups I-IV (with Group IV further subdivided into IV-a and IV-b). Collinearity analysis with other species provided insights into the evolution of BnCNGC . By homology modeling, we predicted the three-dimensional structure of BnCNGC proteins and analyzed cis -acting elements in their promoters, revealing diverse roles in hormone regulation, growth, and stress response. Notably, overexpression of BnCNGC57 ( BnaC09g42460D ) significantly increased seed size, possibly through regulating cell proliferation via the MAPK signaling pathway. Our findings contribute to a better understanding of the BnCNGC gene family and highlight the potential regulatory role of BnCNGC57 in the seed development of B. napus .

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32101661 National Natural Science Foundation of China

Keywords: Brassica napus L.; CNGC; genome; seed size

0 (Cyclic Nucleotide-Gated Cation Channels)
0 (Plant Proteins)

Date Created: 20241109 Date Completed: 20241109 Latest Revision: 20241116

20241116

PMC11545589

10.3390/ijms252111359

39518912