Immunoglobulin secretion influences the composition of chicken caecal microbiota.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Chickens*/microbiology ; Chickens*/immunology ; Cecum*/microbiology ; Immunoglobulins* ; Gastrointestinal Microbiome* ; RNA, Ribosomal, 16S*/genetics; Animals ; Bacteria/classification ; Bacteria/genetics

The chicken caecum is colonised by hundreds of different bacterial species. Which of these are targeted by immunoglobulins and how immunoglobulin expression shapes chicken caecal microbiota has been addressed in this study. Using cell sorting followed by sequencing of V3/V4 variable region of 16S rRNA, bacterial species with increased or decreased immunoglobulin coating were determined. Next, we determined also caecal microbiota composition in immunoglobulin knockout chickens. We found that immunoglobulin coating was common and major taxa were coated with immunoglobulins. Similarly, more taxa required immunoglobulin production for caecum colonisation compared to those which became abundant in immunoglobulin-deficient chickens. Taxa with low immunoglobulin coating such as Lactobacillus, Blautia, [Eubacterium] hallii, Megamonas, Fusobacterium and Desulfovibrio all encode S-layer proteins which may reduce interactions with immunoglobulins. Although there were taxa which overgrew in Ig-deficient chickens (e.g. Akkermansia) indicating immunoglobulin production acted to exclude them from the chicken caecum, in most of the cases, immunoglobulin production more likely contributed to fixing the desired microbiota in the chicken caecum.
(© 2024. The Author(s).)

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QK22020066 Ministerstvo Zemědělství; QK22020066 Ministerstvo Zemědělství; QK22020066 Ministerstvo Zemědělství; QK22020066 Ministerstvo Zemědělství; QK22020066 Ministerstvo Zemědělství; QK22020066 Ministerstvo Zemědělství

0 (Immunoglobulins)
0 (RNA, Ribosomal, 16S)

Date Created: 20241025 Date Completed: 20241026 Latest Revision: 20241028

20241028

PMC11512033

10.1038/s41598-024-76856-2

39455845