Microbial community acclimation during anaerobic digestion of high-oil food waste.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Methane*/metabolism ; Sewage*/microbiology ; Microbiota*; Anaerobiosis ; RNA, Ribosomal, 16S/genetics ; Bacteria/classification ; Bacteria/metabolism ; Bacteria/genetics ; Fatty Acids, Volatile/metabolism ; High-Throughput Nucleotide Sequencing ; Acclimatization ; Food ; Archaea/metabolism ; Archaea/genetics ; Archaea/classification ; Oils/metabolism ; Biodegradation, Environmental ; Food Loss and Waste

Anaerobic digestion is one of the most promising options for the disposal of biodegradable food waste. However, the relatively high content of oil in food waste inhibits the conversion efficiency of anaerobic digestion because of the accumulation of long-chain fatty acids (LCFAs). In this study, activated anaerobic sludge was acclimated to accommodate high-oil conditions. The methane yield of high-oil food waste digested by the acclimated sludge increased by 24.9% compared to that digested by the raw sludge. To determine the internal changes in the acclimated sludge, the shifts in the microbial communities during the acclimation period were investigated via high-throughput sequencing (HTS) based on the 16 S rRNA gene. The results indicated that Bacteroidetes, Firmicutes, Chloroflexi and Proteobacteria were the dominant bacteria at the phylum level. The acclimation time allows some functional bacterial taxa to proliferate, such as Clostridium and Longilinea, which are able to degrade LCFAs and turn them into small organic molecules that also have nutrient value for other bacteria. Among the archaeal communities, the hydrogenotrophic methanogen Methanobacterium nearly supplanted the acetotrophic methanogen Methanosaeta. The time profiles of volatile fatty acids (VFAs) and pH during this period provided additional evidence for the success of the acclimation. This study demonstrated the effectiveness of acclimation and the dynamic of microbial communities, which further contributed to the management and resource utilization of high-oil food waste.
(© 2024. The Author(s).)

Bioresour Technol. 2018 Jan;247:1047-1058. (PMID: 28965912)
Bioresour Technol. 2019 Jan;272:351-359. (PMID: 30384210)
FEMS Microbiol Ecol. 2007 May;60(2):252-65. (PMID: 17374128)
Bioresour Technol. 2009 Oct;100(20):4588-96. (PMID: 19473835)
Water Res. 2017 Oct 15;123:277-289. (PMID: 28672212)
Water Res. 2013 Jul 1;47(11):3835-44. (PMID: 23648286)
Ann N Y Acad Sci. 2008 Mar;1125:171-89. (PMID: 18378594)
Environ Sci Technol. 2006 Oct 15;40(20):6466-72. (PMID: 17120582)
Water Res. 2020 Mar 15;171:115425. (PMID: 31881499)
Water Res. 2016 Oct 15;103:372-382. (PMID: 27486949)
Sci Total Environ. 2021 Apr 10;764:144219. (PMID: 33421748)
Bioresour Technol. 2016 Sep;216:87-94. (PMID: 27235970)
J Environ Manage. 2020 Aug 15;268:110708. (PMID: 32510442)
Int J Syst Evol Microbiol. 2009 Jul;59(Pt 7):1764-70. (PMID: 19542130)
Trends Microbiol. 2007 Apr;15(4):150-5. (PMID: 17320399)
Water Res. 2022 Aug 1;221:118801. (PMID: 35810635)
Water Res. 2015 Apr 15;73:332-41. (PMID: 25697695)
Waste Manag. 2008;28(6):986-92. (PMID: 18561391)
Water Res. 2012 Oct 15;46(16):5268-78. (PMID: 22841596)
Biotechnol Bioeng. 2004 Nov 20;88(4):502-11. (PMID: 15459909)
Bioresour Technol. 2023 Jan;367:128298. (PMID: 36368484)
Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2299-2306. (PMID: 17911301)
Int J Syst Evol Microbiol. 2020 Nov;70(11):5972-6016. (PMID: 33151140)
Water Res. 2023 Mar 1;231:119657. (PMID: 36709568)
Water Res. 2006 Feb;40(3):553-60. (PMID: 16427112)
Bioresour Technol. 2021 Jul;332:125123. (PMID: 33862385)
Bioresour Technol. 2016 Dec;222:380-387. (PMID: 27744163)
Sci Rep. 2016 Jun 29;6:28810. (PMID: 27353502)
J Biotechnol. 2019 Dec 20;306:32-37. (PMID: 31513836)
Bioresour Technol. 2017 Nov;244(Pt 1):213-224. (PMID: 28779674)
Int J Syst Evol Microbiol. 2006 Jun;56(Pt 6):1331-1340. (PMID: 16738111)

OP0UW79H66 (Methane)
0 (Sewage)
0 (RNA, Ribosomal, 16S)
0 (Fatty Acids, Volatile)
0 (Oils)
0 (Food Loss and Waste)

Date Created: 20241025 Date Completed: 20241025 Latest Revision: 20241028

20250114

PMC11511842

10.1038/s41598-024-77136-9

39455737