Effects of Salt Concentration on a Magnetic Nanoparticle-Based Aggregation Assay with a Tunable Dynamic Range.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101204366 Publication Model: Electronic Cited Medium: Internet ISSN: 1424-8220 (Electronic) Linking ISSN: 14248220 NLM ISO Abbreviation: Sensors (Basel) Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c2000-

Biosensing Techniques*/methods ; Magnetite Nanoparticles*/chemistry ; Biotin*/chemistry ; Streptavidin*/chemistry; Salts/chemistry ; Sodium Chloride/chemistry

Magnetic nanoparticles (MNPs) can be functionalized with antibodies to give them an affinity for a biomarker of interest. Functionalized MNPs (fMNPs) cluster in the presence of a multivalent target, causing a change in their magnetization. Target concentration can be proportional to the 3rd harmonic phase of the fMNP magnetization signal. fMNP clustering can also be induced with salt. Generally, salt can alter the stability of charge stabilized fMNPs causing a change in magnetization that is not proportional to the target concentration. We have developed a model system consisting of biotinylated MNPs (biotin-MNPs) that target streptavidin to study the effects of salt concentration on fMNP-based biosensing in simulated in vivo conditions. We have found that biotin-MNP streptavidin targeting was independent of salt concentration for 0.005x to 1.00x phosphate buffered saline (PBS) solutions. Additionally, we show that our biosensor's measurable concentration range (dynamic range) can be tuned with biotin density. Our results can be leveraged to design an in vivo nanoparticle (NP)-based biosensor with enhanced efficacy in the event of varying salt concentrations.

Environ Sci Technol. 2013 Feb 19;47(4):1853-60. (PMID: 23360522)
Analyst. 2011 Apr 21;136(8):1690-6. (PMID: 21369618)
Analyst. 2016 Dec 19;142(1):11-29. (PMID: 27901132)
Colloids Surf B Biointerfaces. 2019 Jul 1;179:374-381. (PMID: 30999116)
Biosens Bioelectron. 2013 May 15;43:366-71. (PMID: 23356994)
ACS Omega. 2021 Feb 26;6(9):6274-6283. (PMID: 33718717)
Sensors (Basel). 2022 Sep 04;22(17):. (PMID: 36081147)
Nanoscale Res Lett. 2013 Sep 08;8(1):381. (PMID: 24011350)
J Control Release. 2016 Aug 10;235:337-351. (PMID: 27297779)
Anal Methods. 2023 Nov 2;15(42):5564-5576. (PMID: 37861233)
J Environ Qual. 2010 Nov-Dec;39(6):1909-24. (PMID: 21284288)
ACS Appl Mater Interfaces. 2020 Mar 25;12(12):13686-13697. (PMID: 32150378)
Anal Chem. 2009 Nov 15;81(22):9425-32. (PMID: 19803497)
Biomacromolecules. 2005 Mar-Apr;6(2):818-24. (PMID: 15762646)
Adv Drug Deliv Rev. 2019 Mar 15;143:22-36. (PMID: 31158406)
ACS Appl Mater Interfaces. 2016 Sep 7;8(35):22808-18. (PMID: 27526263)
J Am Chem Soc. 2003 Aug 27;125(34):10192-3. (PMID: 12926940)
Langmuir. 2011 Jul 19;27(14):8659-64. (PMID: 21644514)
ACS Nano. 2012 Jan 24;6(1):629-40. (PMID: 22136457)
Crit Rev Biomed Eng. 2014;42(1):85-93. (PMID: 25271360)
Nanotechnology. 2013 Sep 6;24(35):355101. (PMID: 23940104)
Biosens Bioelectron. 2013 Dec 15;50:441-6. (PMID: 23896525)
Nanomedicine. 2013 Feb;9(2):194-201. (PMID: 22687896)
ACS Appl Mater Interfaces. 2021 Sep 22;13(37):44136-44146. (PMID: 34499464)
Anal Chem. 2018 Feb 6;90(3):1506-1510. (PMID: 29300471)
Environ Sci Technol. 2009 Dec 1;43(23):8834-9. (PMID: 19943654)
Sensors (Basel). 2023 Apr 30;23(9):. (PMID: 37177614)
Anal Chem. 2020 Jan 7;92(1):1455-1462. (PMID: 31818106)
Nanomedicine. 2009 Jun;5(2):106-17. (PMID: 19071065)
Biomaterials. 2016 Jan;75:295-304. (PMID: 26513421)
IEEE Trans Biomed Eng. 2017 May;64(5):972-979. (PMID: 27352362)
ACS Sens. 2021 Mar 26;6(3):976-984. (PMID: 33496572)

Keywords: aggregation assay; biosensor; magnetic particle spectroscopy; tunable dynamic range

0 (Magnetite Nanoparticles)
6SO6U10H04 (Biotin)
9013-20-1 (Streptavidin)
0 (Salts)
451W47IQ8X (Sodium Chloride)

Date Created: 20241016 Date Completed: 20241016 Latest Revision: 20241019

20241019

PMC11478407

10.3390/s24196241

39409281