Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Schizosaccharomyces*/metabolism ; Schizosaccharomyces*/genetics ; Meiosis* ; Proteomics*/methods ; Schizosaccharomyces pombe Proteins*/metabolism ; Schizosaccharomyces pombe Proteins*/genetics ; Phosphoproteins*/metabolism ; Phosphoproteins*/genetics; Phosphorylation ; Proteome/metabolism

In eukaryotes, chromosomal DNA is equally distributed to daughter cells during mitosis, whereas the number of chromosomes is halved during meiosis. Despite considerable progress in understanding the molecular mechanisms that regulate mitosis, there is currently a lack of complete understanding of the molecular mechanisms regulating meiosis. Here, we took advantage of the fission yeast Schizosaccharomyces pombe, for which highly synchronous meiosis can be induced, and performed quantitative proteomics and phosphoproteomics analyses to track changes in protein expression and phosphorylation during meiotic divisions. We compared the proteomes and phosphoproteomes of exponentially growing mitotic cells with cells harvested around meiosis I, or meiosis II in strains bearing either the temperature-sensitive pat1-114 allele or conditional ATP analog-sensitive pat1-as2 allele of the Pat1 kinase. Comparing pat1-114 with pat1-as2 also allowed us to investigate the impact of elevated temperature (25 °C versus 34 °C) on meiosis, an issue that sexually reproducing organisms face due to climate change. Using TMTpro 18plex labeling and phosphopeptide enrichment strategies, we performed quantification of a total of 4673 proteins and 7172 phosphosites in S. pombe. We found that the protein level of 2680 proteins and the rate of phosphorylation of 4005 phosphosites significantly changed during progression of S. pombe cells through meiosis. The proteins exhibiting changes in expression and phosphorylation during meiotic divisions were represented mainly by those involved in the meiotic cell cycle, meiotic recombination, meiotic nuclear division, meiosis I, centromere clustering, microtubule cytoskeleton organization, ascospore formation, organonitrogen compound biosynthetic process, carboxylic acid metabolic process, gene expression, and ncRNA processing, among others. In summary, our findings provide global overview of changes in the levels and phosphorylation of proteins during progression of S. pombe cells through meiosis at normal and elevated temperatures, laying the groundwork for further elucidation of the functions and importance of specific proteins and their phosphorylation in regulating meiotic divisions in this yeast.
(© 2024. The Author(s).)

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2/0021/22 Slovak Grant Agency VEGA; P30516 Austrian Science Fund; 313011W428 Operational Program Integrated Infrastructure; P 30516 Austria FWF_ Austrian Science Fund FWF; APVV-20-0141 Slovak Research and Development Agency

Keywords: Schizosaccharomyces pombe; Elevated temperature; Meiosis; Pat1 protein kinase; Phosphoproteome; Proteome; Quantitative mass spectrometry

0 (Schizosaccharomyces pombe Proteins)
0 (Phosphoproteins)
0 (Proteome)

Date Created: 20241004 Date Completed: 20241004 Latest Revision: 20241211

20250114

PMC11452395

10.1038/s41598-024-74523-0

39367033