Development of novel canine phage display-derived neutralizing monoclonal antibody fragments against rabies virus from immunized dogs.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Rabies virus*/immunology ; Antibodies, Neutralizing*/immunology ; Single-Chain Antibodies*/immunology ; Single-Chain Antibodies*/genetics ; Rabies*/prevention & control ; Rabies*/immunology ; Antibodies, Monoclonal*/immunology ; Antibodies, Viral*/immunology; Animals ; Dogs ; Peptide Library ; Dog Diseases/immunology ; Dog Diseases/virology ; Dog Diseases/prevention & control ; Dog Diseases/therapy ; Molecular Docking Simulation ; Cell Surface Display Techniques ; Immunization

Animal rabies is a potentially fatal infectious disease in mammals, especially dogs. Currently, the number of rabies cases in pet dogs is increasing in several regions of Thailand. However, no passive postexposure prophylaxis (PEP) has been developed to combat rabies infection in animals. As monoclonal antibodies (MAbs) are promising biological therapies for postinfection, we developed a canine-neutralizing MAb against rabies virus (RABV) via the single-chain variable fragment (scFv) platform. Immunized phage-displaying scFv libraries were constructed from PBMCs via the pComb3XSS system. Diverse canine VHVLκ and VHVLλ libraries containing 2.4 × 10 ⁸ and 1.3 × 10 ⁶ clones, respectively, were constructed. Five unique clones that show binding affinity with the RABV glycoprotein were then selected, of which K9RABVscFv1 and K9RABVscFv16 showed rapid fluorescent foci inhibition test (RFFIT) neutralizing titers above the human protective level of 0.5 IU/ml. Finally, in silico docking predictions revealed that the residues on the CDRs of these neutralizing clones interact mainly with similar antigenic sites II and III on the RABV glycoprotein. These candidates may be used to develop complete anti-RABV MAbs as a novel PEP protocol in pet dogs and other animals.
(© 2024. The Author(s).)

J Immunol Methods. 2008 Mar 20;332(1-2):92-102. (PMID: 18242637)
Nat Commun. 2020 Jan 30;11(1):596. (PMID: 32001700)
Cold Spring Harb Protoc. 2011 Sep 01;2011(9):. (PMID: 21880817)
PLoS Negl Trop Dis. 2015 Apr 16;9(4):e0003709. (PMID: 25881058)
Vaccine. 2021 Sep 24;39(40):5822-5830. (PMID: 34483020)
Cell Discov. 2021 Jul 27;7(1):57. (PMID: 34315862)
J Mol Biol. 2004 Oct 22;343(3):685-701. (PMID: 15465055)
Biotechniques. 1989 Apr;7(4):360-6. (PMID: 2629848)
BMC Biotechnol. 2012 Nov 15;12:87. (PMID: 23153214)
Biotechnology (N Y). 1995 Mar;13(3):255-60. (PMID: 9634767)
PLoS Negl Trop Dis. 2016 Aug 02;10(8):e0004902. (PMID: 27483431)
J Immunol Methods. 1999 Dec 10;231(1-2):177-89. (PMID: 10648937)
Vaccine. 2013 Dec 2;31(49):5897-902. (PMID: 24120673)
Vet Immunol Immunopathol. 2010 Sep 15;137(1-2):64-75. (PMID: 20483487)
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Mar;20(2):243-7. (PMID: 15191736)
Microbiol Immunol. 2005;49(4):311-22. (PMID: 15840956)
Hum Vaccin Immunother. 2022 Dec 31;18(1):2026713. (PMID: 35172707)
J Immunol Methods. 1995 Oct 12;186(1):125-35. (PMID: 7561141)
PLoS Negl Trop Dis. 2009 Nov 03;3(11):e542. (PMID: 19888334)
J Virol. 1988 Jan;62(1):1-7. (PMID: 2446011)
J Mol Biol. 2005 Apr 8;347(4):773-89. (PMID: 15769469)
Biotechnol Res Int. 2011;2011:652147. (PMID: 22007309)
Vaccine. 2008 Nov 5;26(47):5922-7. (PMID: 18804136)
Clin Infect Dis. 2018 Jan 18;66(3):387-395. (PMID: 29020321)
FEBS Lett. 1999 Jun 18;453(1-2):164-8. (PMID: 10403395)
J Biotechnol. 2009 Dec;144(4):253-8. (PMID: 19818816)
Comp Immunol Microbiol Infect Dis. 2013 May;36(3):249-61. (PMID: 23260376)
J Biol Chem. 2000 Nov 17;275(46):36073-8. (PMID: 10969070)
PLoS Negl Trop Dis. 2021 Dec 1;15(12):e0009980. (PMID: 34851953)
Biochemistry. 1998 Sep 15;37(37):12918-26. (PMID: 9737871)
Vet Immunol Immunopathol. 2011 Jan;139(1):27-40. (PMID: 20817275)
Prev Vet Med. 2007 Mar 17;78(3-4):325-32. (PMID: 17141896)
Int J Mol Sci. 2022 Oct 26;23(21):. (PMID: 36361757)
PLoS One. 2021 Sep 1;16(9):e0256779. (PMID: 34469480)
Microbiol Immunol. 2008 Feb;52(2):89-93. (PMID: 18380806)
Virology. 1989 Sep;172(1):206-12. (PMID: 2505450)
Adv Exp Med Biol. 1993;329:611-6. (PMID: 8379434)
Cell Host Microbe. 2020 Mar 11;27(3):441-453.e7. (PMID: 32004500)
Front Vet Sci. 2021 Aug 19;8:699352. (PMID: 34490393)
Sci Rep. 2022 Mar 31;12(1):5449. (PMID: 35361822)
Clin Exp Immunol. 2001 Jul;125(1):94-101. (PMID: 11472431)
Sci Adv. 2022 Jun 17;8(24):eabp9151. (PMID: 35714192)
Viruses. 2021 Nov 19;13(11):. (PMID: 34835117)
Virology. 1987 Nov;161(1):29-36. (PMID: 3672933)
EMBO Mol Med. 2016 Mar 31;8(4):407-21. (PMID: 26992832)
J Virol. 1985 Mar;53(3):926-34. (PMID: 2579247)
PLoS One. 2011 May 09;6(5):e19848. (PMID: 21573024)
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8910-5. (PMID: 9671778)
Biochem Biophys Res Commun. 2010 May 14;395(4):496-501. (PMID: 20382115)
Bing Du Xue Bao. 2016 Jul;32(4):399-403. (PMID: 29979531)
Trop Med Infect Dis. 2020 Feb 21;5(1):. (PMID: 32098049)
Eur J Immunol. 2005 Jul;35(7):2131-45. (PMID: 15971273)
Mol Immunol. 2007 Feb;44(6):1307-15. (PMID: 16814388)
Infect Med (Beijing). 2022 Sep 24;1(4):262-271. (PMID: 38075404)
Bing Du Xue Bao. 2016 Jul;32(4):393-8. (PMID: 29979522)
Appl Microbiol Biotechnol. 2012 Oct;96(2):357-66. (PMID: 22678022)
J Mol Biol. 2003 Jan 10;325(2):325-35. (PMID: 12488098)
MAbs. 2023 Jan-Dec;15(1):2195517. (PMID: 37074212)
PLoS One. 2011 Apr 01;6(4):e17665. (PMID: 21483777)
Mol Immunol. 2014 May;59(1):71-8. (PMID: 24509215)
Front Vet Sci. 2017 Apr 13;4:52. (PMID: 28451589)
PLoS One. 2022 Jul 8;17(7):e0270710. (PMID: 35802654)
Infect Immun. 2002 Aug;70(8):4158-64. (PMID: 12117924)
Vaccine. 2019 Oct 3;37 Suppl 1:A132-A139. (PMID: 30503659)
Zoonoses Public Health. 2012 Nov;59(7):451-67. (PMID: 23180493)
Appl Microbiol Biotechnol. 2022 Feb;106(3):995-1009. (PMID: 35024918)
J Immunol. 1996 Aug 1;157(3):1156-62. (PMID: 8757621)
BMC Biotechnol. 2009 Jan 29;9:6. (PMID: 19175944)
PLoS Negl Trop Dis. 2017 Dec 20;11(12):e0006133. (PMID: 29261658)
Anal Biochem. 2018 Aug 15;555:81-93. (PMID: 29775561)
J Immunol Methods. 2000 Aug 28;242(1-2):159-81. (PMID: 10986398)
PLoS One. 2023 Nov 3;18(11):e0292833. (PMID: 37922253)

PHD60I0075 National Research Council of Thailand; N42A670624 National Research Council of Thailand

0 (Antibodies, Neutralizing)
0 (Single-Chain Antibodies)
0 (Antibodies, Monoclonal)
0 (Antibodies, Viral)
0 (Peptide Library)

Date Created: 20241002 Date Completed: 20241002 Latest Revision: 20241005

20250114

PMC11447112

10.1038/s41598-024-73339-2

39358469