Cationic fluorinated micelles for cell labeling and ¹⁹ F-MR imaging.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Micelles* ; Cations*/chemistry ; Cell Survival*/drug effects; Humans ; Fluorine/chemistry ; Fluorine-19 Magnetic Resonance Imaging/methods ; Contrast Media/chemistry ; Animals ; Magnetic Resonance Imaging/methods ; Halogenation ; Phantoms, Imaging ; Staining and Labeling/methods ; Mice

Magnetic resonance imaging (MRI) relies on appropriate contrast agents, especially for visualizing transplanted cells within host tissue. In recent years, compounds containing fluorine-19 have gained significant attention as MRI probe, particularly in dual ¹ H/ ¹⁹ F-MR imaging. However, various factors affecting probe sensitivity, such as fluorine content and the equivalency of fluorine atoms, must be considered. In this study, we synthesized fluorinated micelles with adjustable surface positive charge density and investigated their physicochemical properties and MRI efficacy in phantoms and labeled cells. While the micelles exhibited clear signals in ¹⁹ F-MR spectra and imaging, the concentrations required for MRI visualization of labeled cells were relatively high, adversely affecting cell viability. Despite their favourable physicochemical properties, achieving higher labeling rates without compromising cell viability during labeling remains a challenge for potential in vivo applications.
(© 2024. The Author(s).)

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IN00023001 Ministerstvo Zdravotnictví Ceské Republiky; LM2018124 Ministerstvo Školství, Mládeže a Tělovýchovy; LX22NPO5104 National Institute for Research of Metabolic and Cardiovascular Diseases; CZ.02.1.01/0.0/0.0/16_013/0001821 European Structural and Investments Funds in the frame of the Research Development and Education

Keywords: 19F magnetic resonance imaging; 19F magnetic resonance spectroscopy; Cell labeling; Fluorinated micelles

0 (Micelles)
0 (Cations)
284SYP0193 (Fluorine)
0 (Contrast Media)

Date Created: 20240930 Date Completed: 20240930 Latest Revision: 20241003

20241003

PMC11442823

10.1038/s41598-024-73511-8

39349687