Prenanthes purpurea and 3,5-DiCQA Alleviate Cellular Stress in H 2 O 2 -Induced Neurotoxicity: An In Vitro Comparative Study.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Hydrogen Peroxide*/toxicity ; Oxidative Stress*/drug effects; Plant Extracts/pharmacology ; Plant Extracts/chemistry ; Antioxidants/pharmacology ; Antioxidants/chemistry ; Neuroprotective Agents/pharmacology ; Neuroprotective Agents/chemistry ; Humans ; Quinic Acid/analogs & derivatives ; Quinic Acid/pharmacology ; Quinic Acid/chemistry ; Chlorogenic Acid/pharmacology ; Chlorogenic Acid/analogs & derivatives ; Chlorogenic Acid/chemistry ; Animals ; Cell Survival/drug effects

Oxidative stress exerts multiple disruptive effects on cellular morphology and function and is a major detriment to age-related and pathological neurodegenerative processes. The present study introduces an evaluative and comparative investigation of the antioxidant and cytoprotective properties of a Prenanthes purpurea extract and its major constituent 3,5-dicaffeoylquinic acid (DiCQA) in an in vitro model of H 2 O 2 -induced neurotoxicity. Using validated in vitro and in silico approaches, we established the presence and concentration dynamics of cellular protection in a 24 h pretreatment regimen with the natural products. The conducted cytotoxicity studies and the automated Chou-Talalay analysis for studying drug interactions demonstrated a strong antagonistic effect of the tested substances against oxidative stimuli in an "on demand" manner, prevailing at the higher end of the concentration range. These findings were further supported by the proteomic characterization of the treatment samples, accounting for a more distinct neuroprotection provided by the pure polyphenol 3,5-DiCQA.

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BG-RRP-2.004-0004-C01. European Union's Next Generation EU economic recovery package via the National Recovery and Resilience Plan of the Republic of Bulgaria

Keywords: DiCQA; Prenanthes purpurea; neuroprotection; neurotoxicity; oxidative stress; polyphenols

BBX060AN9V (Hydrogen Peroxide)
0 (Plant Extracts)
0 (Antioxidants)
0 (Neuroprotective Agents)
058C04BGYI (Quinic Acid)
318ADP12RI (Chlorogenic Acid)
0 (caffeoylquinic acid)

Date Created: 20240928 Date Completed: 20240928 Latest Revision: 20240930

20250114

PMC11431944

10.3390/ijms25189805

39337291