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Contrast enhanced longitudinal changes observed in an experimental bleomycin-induced lung fibrosis rat model by radial DCE-MRI at 9.4T.

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  • معلومة اضافية
    • Corporate Authors:
      TRISTAN Consortium
    • المصدر:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: San Francisco, CA : Public Library of Science
    • الموضوع:
      Bleomycin* ; Magnetic Resonance Imaging*/methods ; Pulmonary Fibrosis*/chemically induced ; Pulmonary Fibrosis*/diagnostic imaging ; Pulmonary Fibrosis*/pathology ; Rats, Sprague-Dawley* ; Contrast Media* ; Disease Models, Animal*; Animals ; Male ; Rats ; Lung/diagnostic imaging ; Lung/pathology ; Lung/drug effects
    • نبذة مختصرة :
      Competing Interests: The authors have declared that no competing interests exist.
      Identifying biomarkers in fibrotic lung disease is key for early anti-fibrotic intervention. Dynamic contrast-enhanced (DCE) MRI offers valuable perfusion-related insights in fibrosis but adapting human MRI methods to rodents poses challenges. Here, we explored these translational challenges for the inflammatory and fibrotic phase of a bleomycin lung injury model in rats. Eleven male Sprague-Dawley rats received a single intratracheal dose of bleomycin (1000iU), four control rats received saline. Imaging was performed on days 7 and 28 post-induction. Ultra-short echo time imaging was used to image the lung for 7 minutes after which Clariscan was injected intravenously. Lung signal changes were measured for an additional 21 minutes. Images were reconstructed with a sliding-window approach, providing a temporal resolution of 10 seconds per image. After imaging on day 28, animals were euthanized, and lungs were collected for histology. Bleomycin-exposed rats initially exhibited reduced body weight, recovering to control levels after 20 days. Lung volume increased in bleomycin animals from 4.4±0.9 ml in controls to 5.5±0.5 ml and 6.5±1.2 ml on day 7 and 28. DCE-MRI showed no change of initial gradient of relative enhancement in the curves between controls and bleomycin animals on day 7 and 28 post-induction. On day 7, the DCE-MRI washout phase in bleomycin animals had higher signals than the saline group and than observed at a later time point. Lung pixels were binned in 7 enhancement classes. On day 28, the size of low relative enhancement bins almost doubled in volume compared to controls and animals on day 7 post-induction. Histology on day 28 suggests that findings could be explained by changes in lung tissue density due to lung volume increase. Adapting this clinical MRI method to rodents at 9.4T remains a challenge. Future studies may benefit from lower field strength MRI combined with higher temporal resolution DCE-MRI.
      (Copyright: © 2024 in ‘t Zandt et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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    • الرقم المعرف:
      11056-06-7 (Bleomycin)
      0 (Contrast Media)
    • الموضوع:
      Date Created: 20240927 Date Completed: 20240927 Latest Revision: 20240929
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC11432896
    • الرقم المعرف:
      10.1371/journal.pone.0310643
    • الرقم المعرف:
      39331604