Green synthesis of silver and gold-doped zinc oxide nanocomposite with propolis extract for enhanced anticancer activity.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Propolis*/chemistry ; Propolis*/pharmacology ; Gold*/chemistry ; Nanocomposites*/chemistry ; Silver*/chemistry ; Zinc Oxide*/chemistry ; Zinc Oxide*/pharmacology ; Antineoplastic Agents*/pharmacology ; Antineoplastic Agents*/chemistry ; Antineoplastic Agents*/chemical synthesis ; Green Chemistry Technology*; Humans ; Hep G2 Cells ; MCF-7 Cells ; Metal Nanoparticles/chemistry ; Cell Survival/drug effects

Metal and metal oxide nanocomposites have unique properties and are promising for antibacterial and anticancer applications. In this work, we aimed to highlight the relationship between the biosynthesis ways of silver and gold-doped zinc oxide nanocomposites and their functions as anticancer on cell lines (MCF-7 and HepG2). The propolis was used to biosynthesize four different nanoparticles with the same components, including zinc, gold and silver. The nanocomposites were characterized using various techniques, including ultraviolet-visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Energy Dispersive X-ray analysis (EDX) and cytotoxicity assays. The result of this study showed that formed nanocomposites have a similar level of Zn, Au, and Ag, ranging from 23-34%, 2-6%, and 2-3%, respectively. In addition, adding the components simultaneously produces the fastest color change, and the fabricated nanoparticles have spherical shapes with different layers. In addition, the prepared nanoparticles influenced the cell viability of the cancer cell lines, with the most effective one when Zn, Au, and Ag were added spontaneously to form a nanocomposite called (All) with IC 50 of 24.5 µg/mL for MCF7 cells and 29.1 µg/mL for HepG2 cells. Thus, the study illustrates that the preparation of nanocomposite generated through green synthesis with different methods significantly affects the structure and function and may improve the synthesis of nanocomposite to be developed into an efficacious therapeutic agent for cancers. In addition, this study opens the door toward a novel track in the field of nanocomposites as it links the synthesis with structure and function. Further anti-cancer properties, as well as animal testing are needed for those nanocomposites.
(© 2024. The Author(s).)

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41909 Science and Technology Development Fund

Keywords: Anticancer; Gold nanoparticle; Nanocomposite; Silver nanoparticle; Zinc oxide nanoparticle

9009-62-5 (Propolis)
7440-57-5 (Gold)
3M4G523W1G (Silver)
SOI2LOH54Z (Zinc Oxide)
0 (Antineoplastic Agents)

Date Created: 20240918 Date Completed: 20240918 Latest Revision: 20240921

20240922

PMC11410827

10.1038/s41598-024-71758-9

39294193