Protective Effects of Vitamin D on Proteoglycans of Human Articular Chondrocytes through TGF-β1 Signaling.

Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI Publishing

Chondrocytes*/metabolism ; Chondrocytes*/drug effects ; Signal Transduction*/drug effects ; Transforming Growth Factor beta1*/metabolism ; Aggrecans*/metabolism ; Tumor Necrosis Factor-alpha*/metabolism ; Vitamin D*/pharmacology ; Proteoglycans*/metabolism ; Proteoglycans*/pharmacology ; Cartilage, Articular*/metabolism ; Cartilage, Articular*/drug effects; Humans ; Cells, Cultured ; Cell Survival/drug effects ; Osteoarthritis/metabolism ; Receptor, Transforming Growth Factor-beta Type I/metabolism ; Receptors, Calcitriol/metabolism

The extracellular matrix of cartilage primarily constitutes of collagen and aggrecan. Cartilage degradation starts with aggrecan loss in osteoarthritis (OA). Vitamin D (VD) plays an essential role in several inflammation-related diseases and can protect the collagen in cartilage during OA. The present study focused on the role of VD in aggrecan turnover of human articular chondrocytes treated with tumor necrosis factor α (TNF-α) and the possible mechanism. Treatment with different doses of VD and different periods of intervention with TNF-α and TGF-β1 receptor (TGFβR1) inhibitor SB525334 were investigated. The viability of human chondrocytes and extracellular secretion of TGF-β1 were measured. The expression of intracellular TGFβR1 and VD receptor was examined. Transcriptional and translational levels of aggrecan and the related metabolic factors were analyzed. The results showed that TNF-α markedly reduced the viability, TGFβR1 expressions and aggrecan levels of human chondrocytes, and increased disintegrin and metalloproteinase with thrombospondin motifs. The alterations were partially inhibited by VD treatment. Furthermore, the effects of VD were blocked by the TGFβR1 inhibitor SB525334 in TNF-α-treated cells. VD may prevent proteoglycan loss due to TNF-α via TGF-β1 signaling in human chondrocytes.

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12172011 National Natural Science Foundation of China; 11872076 National Natural Science Foundation of China

Keywords: TGF-β1 signaling; TNF-α; osteoarthritis; proteoglycan; vitamin D

0 (Transforming Growth Factor beta1)
0 (Aggrecans)
0 (Tumor Necrosis Factor-alpha)
1406-16-2 (Vitamin D)
0 (Proteoglycans)
0 (TGFB1 protein, human)
EC 2.7.11.30 (Receptor, Transforming Growth Factor-beta Type I)
EC 2.7.11.30 (TGFBR1 protein, human)
0 (Receptors, Calcitriol)
0 (ACAN protein, human)

Date Created: 20240914 Date Completed: 20240914 Latest Revision: 20240924

20240924

PMC11396982

10.3390/nu16172991

39275306