Cloning and Functional Study of AmGDSL1 in Agropyron mongolicum .

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Cloning, Molecular* ; Gene Expression Regulation, Plant* ; Agropyron*/genetics ; Agropyron*/metabolism ; Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Nicotiana*/genetics ; Nicotiana*/metabolism; Droughts ; Stress, Physiological/genetics ; Plants, Genetically Modified/genetics ; Plant Roots/genetics ; Plant Roots/metabolism ; Lipase/metabolism ; Lipase/genetics

Agropyron mongolicum Keng is a diploid perennial grass of triticeae in gramineae. It has strong drought resistance and developed roots that can effectively fix the soil and prevent soil erosion. GDSL lipase or esterases/lipase has a variety of functions, mainly focusing on plant abiotic stress response. In this study, a GDSL gene from A. mongolicum , designated as AmGDSL1 , was successfully cloned and isolated. The subcellular localization of the AmGDSL1 gene (pCAMBIA1302- AmGDSL1 -EGFP) results showed that the AmGDSL1 protein of A. mongolicum was only localized in the cytoplasm. When transferred into tobacco ( Nicotiana benthamiana ), the heterologous expression of AmGDSL1 led to enhanced drought tolerance. Under drought stress, AmGDSL1 overexpressing plants showed fewer wilting leaves, longer roots, and larger root surface area. These overexpression lines possessed higher superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and proline (PRO) activities. At the same time, the malondialdehyde (MDA) content was lower than that in wild-type (WT) tobacco. These findings shed light on the molecular mechanisms involved in the GDSL gene's role in drought resistance, contributing to the discovery and utilization of drought-resistant genes in A. mongolicum for enhancing crop drought resistance.

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No.32360338 National Natural Science Foundation of China

Keywords: Agropyron mongolicum; AmGDSL1 gene; antioxidant activity; drought resistance

0 (Plant Proteins)
EC 3.1.1.3 (Lipase)

Date Created: 20240914 Date Completed: 20240914 Latest Revision: 20240916

20240916

PMC11395167

10.3390/ijms25179467

39273413