PEG-SeNPs as therapeutic agents inhibiting apoptosis and inflammation of cells infected with H1N1 influenza A virus.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Influenza A Virus, H1N1 Subtype*/drug effects ; Apoptosis*/drug effects ; Polyethylene Glycols*/chemistry ; Polyethylene Glycols*/pharmacology; Animals ; Dogs ; Madin Darby Canine Kidney Cells ; Inflammation/drug therapy ; Antiviral Agents/pharmacology ; Selenium/pharmacology ; Selenium/chemistry ; Reactive Oxygen Species/metabolism ; Nanoparticles/chemistry ; Humans ; DNA Damage/drug effects

The rapid variation of influenza challenges vaccines and treatments, which makes an urgent task to develop the high-efficiency and low-toxicity new anti-influenza virus drugs. Selenium is one of the essential trace elements for the human body that possesses a good antiviral activity. In this study, we assessed anti-influenza A virus (H1N1) activity of polyethylene glycol (PEG)-modified gray selenium nanoparticles (PEG-SeNPs) on Madin-Darby Canine Kidney (MDCK) cells in vitro. CCK-8 assay showed that PEG-SeNPs had a protective effect on H1N1-infected MDCK cells. Moreover, PEG-SeNPs significantly reduced the mRNA level of H1N1. TUNEL-DAPI test showed that DNA damage reached a high level but effectively prevented after PEG-SeNPs treatment. Meanwhile, JC-1, Annexin V-FITC and cell cycle assay demonstrated the apoptosis induced by H1N1 was reduced greatly when treated with PEG-SeNPs. Furthermore, the downregulation of p-ATM, p-ATR and P53 protein, along with the upregualation of AKT protein indicated that PEG-SeNPs could inhibit H1N1-induced cell apoptosis through reactive oxygen species (ROS)-mediated related signaling pathways. Finally, Cytokine detection demonstrated PEG-SeNPs inhibited the production of pro-inflammatory factors after infection, including IL-1β, IL-5, IL-6, and TNF-α. To sum up, PEG-SeNPs might become a new potential anti-H1N1 influenza virus drug due to its antiviral and anti-inflammatory activity.
(© 2024. The Author(s).)

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02-408-2203-2080, 02-408-2304-19080XM and 02-408-240603131099 Guangzhou Medical University Students' Scientific Research In-novation Ability Improvement Project; 2020A1515110648 Guangdong Natural Science Foundation; 202201020628 tech-nology planning projects of Guangzhou

Keywords: Apoptosis; Cytokine; Influenza virus; PEG-SeNPs; Signaling pathways

3WJQ0SDW1A (Polyethylene Glycols)
0 (Antiviral Agents)
H6241UJ22B (Selenium)
0 (Reactive Oxygen Species)

Date Created: 20240912 Date Completed: 20240912 Latest Revision: 20240916

20250114

PMC11393426

10.1038/s41598-024-71486-0

39266597