Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Association between real-time polymerase chain reaction cycle threshold value and clinical presentation in children with Bordetella pertussis.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101510759 Publication Model: Electronic Cited Medium: Internet ISSN: 1824-7288 (Electronic) Linking ISSN: 17208424 NLM ISO Abbreviation: Ital J Pediatr Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : BioMed Central
    • الموضوع:
      Real-Time Polymerase Chain Reaction* ; Whooping Cough*/diagnosis ; Whooping Cough*/microbiology ; Bordetella pertussis*/genetics ; Bordetella pertussis*/isolation & purification; Humans ; Male ; Female ; Child, Preschool ; Child ; Infant ; China ; Nasopharynx/microbiology ; Adolescent
    • نبذة مختصرة :
      Background: The cycle threshold (Ct) value is inversely proportional to the number of copies of the target region in a sample, suggesting that a low Ct value indicates a high pathogen load. The relationship between Ct value and clinical presentation in children with pertussis is not well-defined.
      Methods: We investigated the relationships between the Ct value of nasopharyngeal samples positive for Bordetella pertussis deoxyribonucleic acid via real-time polymerase chain reaction (PCR), collected from children on admission and their adult family members between May 2022 and March 2024 at Hangzhou Children's Hospital, China. The study focused on the correlation between Ct value and clinical presentation in children with pertussis.
      Results: The Ct value was positively correlated with age (r = 0.362, P = 0.001). The mean Ct value for children with pertussis was 28.0 (range: 22.0-32.0), which was lower than the 32.0 (range: 30.0-34.0) observed in adults. Ct value was inversely correlated with length of stay, an indicator of disease severity (r = -0.356, P = 0.001). Logistic regression analyses revealed that both Ct value (OR: 0.891, 95% CI: 0.799-0.993, P = 0.036) and white blood cell count (OR: 1.127, 95% CI: 1.005-1.263, P = 0.040) were independently associated with severity of pertussis.
      Conclusions: Real-time PCR Ct values at initial diagnosis for pertussis may potentially predict severe disease outcomes in children.
      (© 2024. The Author(s).)
    • References:
      Pediatr Crit Care Med. 2017 Jan;18(1):e42-e47. (PMID: 27811532)
      J Infect. 2016 Apr;72(4):460-7. (PMID: 26850358)
      Diagn Microbiol Infect Dis. 2013 May;76(1):10-5. (PMID: 23490009)
      Pediatrics. 2015 Oct;136(4):635-41. (PMID: 26347437)
      J Microbiol Immunol Infect. 2021 Aug;54(4):687-692. (PMID: 32513618)
      J Pediatric Infect Dis Soc. 2013 Mar;2(1):1-6. (PMID: 26619437)
      PLoS One. 2015 Jul 17;10(7):e0133209. (PMID: 26186564)
      Infect Dis Ther. 2021 Mar;10(1):335-346. (PMID: 33270206)
      Eur J Clin Microbiol Infect Dis. 2022 Jan;41(1):9-20. (PMID: 34398346)
      Clin Microbiol Infect. 2011 Mar;17(3):365-70. (PMID: 20456454)
      Pathog Dis. 2016 Oct;74(7):. (PMID: 27609461)
      Clin Infect Dis. 2008 Aug 1;47(3):328-38. (PMID: 18558873)
      Epidemiol Infect. 2019 Jan;147:e184. (PMID: 31063110)
      Clin Infect Dis. 2016 Dec 1;63(suppl 4):S134-S141. (PMID: 27838665)
      Lancet Infect Dis. 2017 Sep;17(9):974-980. (PMID: 28623146)
      J Infect Dis. 2021 Sep 30;224(12 Suppl 2):S310-S320. (PMID: 34590129)
      Vaccines (Basel). 2023 Apr 24;11(5):. (PMID: 37242993)
      J Pediatr. 2017 Sep;188:217-223.e1. (PMID: 28622957)
      BMC Infect Dis. 2020 Nov 16;20(1):852. (PMID: 33198647)
      Pediatr Infect Dis J. 2022 May 1;41(5):388-393. (PMID: 35093999)
      Vaccine. 2021 Mar 19;39(12):1687-1692. (PMID: 33642160)
      Biomed Environ Sci. 2017 Jul;30(7):545-548. (PMID: 28756816)
      Am J Trop Med Hyg. 2019 Jun;100(6):1342-1354. (PMID: 30994096)
      Oman Med J. 2022 May 31;37(3):e372. (PMID: 35712375)
      Infect Dis Ther. 2021 Sep;10(3):1115-1140. (PMID: 33928533)
      J Antimicrob Chemother. 2021 Sep 23;76(Suppl 3):iii33-iii49. (PMID: 34555159)
      J Infect. 2024 Apr;88(4):106140. (PMID: 38513738)
      Medicine (Baltimore). 2017 Dec;96(48):e8823. (PMID: 29310361)
      Pediatr Infect Dis J. 2011 Feb;30(2):161-3. (PMID: 20811313)
      Clin Infect Dis. 2014 Jun;58(11):1523-9. (PMID: 24633685)
      BMC Infect Dis. 2017 Jul 12;17(1):492. (PMID: 28701160)
      PLoS One. 2018 Apr 13;13(4):e0195979. (PMID: 29652945)
    • Grant Information:
      2023KY202 Project of Zhejiang Province Medical and Health Technology Plan
    • Contributed Indexing:
      Keywords: Cycle threshold; Pertussis; Polymerase chain reaction
    • الموضوع:
      Date Created: 20240910 Date Completed: 20240911 Latest Revision: 20240914
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC11389257
    • الرقم المعرف:
      10.1186/s13052-024-01753-3
    • الرقم المعرف:
      39256818