Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Histological methods for plant tissues.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Historical Metallurgy Society Country of Publication: England NLM ID: 8003807 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2046-0236 (Electronic) Linking ISSN: 01478885 NLM ISO Abbreviation: J Histotechnol Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2020- : Bedfordshire : Historical Metallurgy Society
      Original Publication: [Downers Grove, IL : Mayo Publications]
    • الموضوع:
      Paraffin Embedding*/methods ; Tissue Fixation*/methods; Plants ; Staining and Labeling/methods ; Phenazines ; Histological Techniques/methods ; Formaldehyde/pharmacology ; Plant Leaves ; Frozen Sections/methods ; Flowers ; Plant Roots
    • نبذة مختصرة :
      Although many of the structures and organelles of vegetative cells are comparable to those of animal tissues, significant differences between the two kingdoms require modifications in histological techniques for both tissue processing steps and histochemical staining techniques. The authors investigated the challenges of working with plant tissues by collecting various flora to represent the four main plant organs: leaf, stem, root, and flower/fruit. Triplicate samples for each specimen were placed into formalin for paraffin embedding, placed into formalin for later frozen sections, and used fresh to undergo immediate frozen sectioning. Frozen sections of plant tissues were more difficult to obtain than formalin-fixed paraffin-embedded (FFPE) sections, exhibited tissue loss during staining, and were inferior morphologically to FFPE sections. Although, historically, plant tissue fixation and processing has employed several different reagents compared with those used in animal tissue processing and took significantly longer times, the current investigation determined reagents and protocols from a modern histology laboratory which processes mammalian tissues can be applied to plant tissue processing with only slight modifications in respect to reagent timing. Additionally, staining techniques were compared and while it is well known that plant cell walls stain well with safranin O, the current investigation determined the uptake of safranin O can be accelerated by incubating at 60°C.
    • Contributed Indexing:
      Keywords: Cell wall; histology; leaves; morphology; safranin O; stems; vegetation; wood
    • الرقم المعرف:
      0 (Phenazines)
      XTX0YXU2HV (safranine T)
      1HG84L3525 (Formaldehyde)
    • الموضوع:
      Date Created: 20240902 Date Completed: 20250225 Latest Revision: 20250225
    • الموضوع:
      20250225
    • الرقم المعرف:
      10.1080/01478885.2024.2397989
    • الرقم المعرف:
      39222359