Stability and Bioaccessibility of Carotenoids from Sea Buckthorn Pomace Encapsulated in Alginate Hydrogel Beads.

Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI Publishing

Alginates*/chemistry ; Hippophae*/chemistry ; Hydrogels*/chemistry ; Carotenoids*/pharmacokinetics ; Carotenoids*/administration & dosage ; Carotenoids*/analysis ; Biological Availability*; Zeaxanthins/chemistry ; Yogurt/analysis ; Sunflower Oil/chemistry ; Humans ; beta Carotene/chemistry ; beta Carotene/pharmacokinetics ; beta Carotene/administration & dosage ; Fruit/chemistry

Carotenoids, the natural pigments that confer the bright orange color of sea buckthorn berries, are also associated with several health benefits, such as antioxidant activity and skin and eye protection. Due to their lipophilic nature and localization, carotenoids are largely retained in the sea buckthorn pomace (SBP) resulting from juice production. Carotenoids from SBP (70.03 mg/100 g DW), extracted and characterized by HPLC-PDA, contained zeaxanthin (free and esterified) and beta-carotene as major compounds. The SBP carotenoids-enriched sunflower oil was further encapsulated in Ca-alginate hydrogel beads (98.4% encapsulation efficiency) using ionotropic gelation. The hydrogel beads were characterized by confocal laser scanning microscopy and scanning electron microscopy. Fairly good stability (>64%) of the encapsulated carotenoids in the alginate hydrogel beads during storage (30 days, 4 °C and 25 °C) was found, with zeaxanthin esters being the most stable compounds, for all the experimental conditions. The bioaccessibility of the total carotenoids (INFOGEST protocol) was 42.1 ± 4.6% from hydrated, and, respectively, 40.8 ± 4% from dehydrated SBP alginate hydrogel beads. The addition of yogurt to the dehydrated hydrogel beads had a positive effect on the bioaccessibility of free and esterified zeaxanthin, but not on that of the carotenes. In conclusion, SBP is a valuable source of carotenoids which can be protected by encapsulation in alginate hydrogel beads, thus still retaining a good bioaccessibility.

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Grant, number 243/2021. This work was supported by PN-III-P4-ID-PCE-2020-1172

Keywords: alginate hydrogel beads; bioaccessibility; sea buckthorn; stability; zeaxanthin

0 (Alginates)
0 (Hydrogels)
36-88-4 (Carotenoids)
0 (Zeaxanthins)
0 (Sunflower Oil)
01YAE03M7J (beta Carotene)

Date Created: 20240829 Date Completed: 20240829 Latest Revision: 20240903

20240903

PMC11357371

10.3390/nu16162726

39203862