Fluorescence Turn-Off Ligand for Parallel G-Quadruplexes.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

G-Quadruplexes* ; Circular Dichroism*; Ligands ; Humans ; Spectrometry, Fluorescence ; Promoter Regions, Genetic ; DNA/chemistry ; DNA/metabolism ; Fluorescence ; Fluorescent Dyes/chemistry

Parallel-stranded G-quadruplex structures are found to be common in the human promoter sequences. We tested highly fluorescent 9-methoxyluminarine ligand (9-MeLM) binding interactions with different parallel G-quadruplexes DNA by spectroscopic methods such as fluorescence and circular dichroism (CD) titration as well as UV melting profiles. The results showed that the studied 9-MeLM ligand interacted with the intramolecular parallel G-quadruplexes (G4s) with similar affinity. The binding constants of 9-methoxyluminarine with different parallel G4s were determined. The studies upon oligonucleotides with different flanking sequences on c- MYC G-quadruplex suggest that 9-methoxyluminarine may preferentially interact with 3'end of the c- MYC promoter. The high decrease in 9-MeLM ligand fluorescence upon binding to all tested G4s indicates that 9-methoxyluminarine molecule can be used as a selective fluorescence turn-off probe for parallel G-quadruplexes.

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Keywords: 9-methoxyluminarine; CD spectroscopy; UV–vis spectroscopy; fluorescence; parallel G-quadruplexes

0 (Ligands)
9007-49-2 (DNA)
0 (Fluorescent Dyes)

Date Created: 20240829 Date Completed: 20240829 Latest Revision: 20240901

20240902

PMC11357100

10.3390/molecules29163907

39202986