Genome-wide identification of R2R3-MYB transcription factor subfamily genes involved in salt stress in rice (Oryza sativa L.).

Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE

Original Publication: London : BioMed Central, [2000-

Oryza*/genetics ; Oryza*/metabolism ; Transcription Factors*/genetics ; Transcription Factors*/metabolism ; Salt Stress*/genetics ; Phylogeny* ; Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Gene Expression Regulation, Plant*; Genome, Plant ; Multigene Family ; Gene Expression Profiling ; Chromosomes, Plant/genetics

Background: R2R3-MYB transcription factors belong to one of the largest gene subfamilies in plants, and they are involved in diverse biological processes. However, the role of R2R3-MYB transcription factor subfamily genes in the response of rice (Oryza sativa L.) to salt stress has been rarely reported.
Results: In this study, we performed a genome-wide characterization and expression identification of rice R2R3-MYB transcription factor subfamily genes. We identified a total of 117 R2R3-MYB genes in rice and characterized their gene structure, chromosomal location, and cis-regulatory elements. According to the phylogenetic relationships and amino acid sequence homologies, the R2R3-MYB genes were divided into four groups. qRT-PCR of the R2R3-MYB genes showed that the expression levels of 10 genes significantly increased after 3 days of 0.8% NaCl treatment. We selected a high expression gene OsMYB2-115 for further analysis. OsMYB2-115 was highly expressed in the roots, stem, leaf, and leaf sheath. OsMYB2-115 was found to be localized in the nucleus, and the yeast hybrid assay showed that OsMYB2-115 has transcriptional activation activity.
Conclusion: This result provides important information for the functional analyses of rice R2R3-MYB transcription factor subfamily genes related to the salt stress response and reveals that OsMYB2-115 may be an important gene associated with salt tolerance in rice.
(© 2024. The Author(s).)

Plant Sci. 2015 Jul;236:146-56. (PMID: 26025528)
Sci Rep. 2015 Jun 05;5:11037. (PMID: 26047035)
Front Plant Sci. 2019 Feb 18;10:168. (PMID: 30833955)
Biology (Basel). 2020 Mar 24;9(3):. (PMID: 32213912)
Plant J. 1999 Sep;19(6):679-89. (PMID: 10571853)
Plant J. 2008 Jan;53(1):53-64. (PMID: 17971045)
Front Plant Sci. 2022 Jun 27;13:934877. (PMID: 35832230)
BMB Rep. 2010 Jan;43(1):34-9. (PMID: 20132733)
Plant Physiol. 2009 Sep;151(1):275-89. (PMID: 19625633)
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. (PMID: 29722887)
Curr Opin Plant Biol. 2001 Oct;4(5):447-56. (PMID: 11597504)
Plants (Basel). 2022 Jun 02;11(11):. (PMID: 35684271)
Int J Biol Macromol. 2021 Nov 30;191:359-376. (PMID: 34534587)
Nucleic Acids Res. 2020 Jan 8;48(D1):D1104-D1113. (PMID: 31701126)
Plant Signal Behav. 2016;11(1):e1117723. (PMID: 26636625)
Plant Biotechnol J. 2023 Jun;21(6):1140-1158. (PMID: 36752420)
Front Plant Sci. 2015 Dec 24;6:1157. (PMID: 26734052)
PLoS One. 2015 Jun 02;10(6):e0127831. (PMID: 26035591)
Plant Physiol Biochem. 2023 Oct;203:108041. (PMID: 37722281)
Bioinformatics. 2015 Apr 15;31(8):1296-7. (PMID: 25504850)
Nucleic Acids Res. 2007 Jan;35(Database issue):D883-7. (PMID: 17145706)
Plant Cell Environ. 2020 Aug;43(8):1925-1943. (PMID: 32406163)
J Exp Bot. 2015 Sep;66(19):5997-6008. (PMID: 26139822)
Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6428-32. (PMID: 1631139)
Trends Plant Sci. 2010 Oct;15(10):573-81. (PMID: 20674465)
Mol Cells. 2011 May;31(5):447-54. (PMID: 21399993)
Nat Struct Biol. 1996 Feb;3(2):178-87. (PMID: 8564545)
Hortic Res. 2023 Apr 21;10(6):uhad080. (PMID: 37323234)
J Genet Genomics. 2009 Jan;36(1):17-29. (PMID: 19161942)
Plant Mol Biol. 1999 Nov;41(5):577-85. (PMID: 10645718)
Mol Plant. 2020 Aug 3;13(8):1194-1202. (PMID: 32585190)
PLoS Genet. 2009 Mar;5(3):e1000440. (PMID: 19325888)
J Exp Bot. 2012 Apr;63(7):2541-56. (PMID: 22301384)
Plant Cell. 2007 Jul;19(7):2264-77. (PMID: 17644729)
Plant Cell Physiol. 2013 Feb;54(2):e6. (PMID: 23299411)
Front Plant Sci. 2022 Jan 03;12:782220. (PMID: 35046974)
Plant Cell Physiol. 2006 Jan;47(1):141-53. (PMID: 16284406)
EMBO J. 1994 Nov 15;13(22):5383-92. (PMID: 7957104)
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W475-8. (PMID: 21470960)
Annu Rev Plant Biol. 2008;59:651-81. (PMID: 18444910)
PLoS One. 2014 Mar 25;9(3):e92913. (PMID: 24667379)
Plants (Basel). 2022 Jul 25;11(15):. (PMID: 35893632)
Front Plant Sci. 2022 Oct 24;13:1022076. (PMID: 36352865)
New Phytol. 2020 Jul;227(2):455-472. (PMID: 32167578)
Trends Plant Sci. 2019 Oct;24(10):934-946. (PMID: 31358471)
Plant J. 2013 Oct;76(2):258-73. (PMID: 23855375)
Int J Mol Sci. 2021 Feb 24;22(5):. (PMID: 33668247)
Plant Cell. 2005 Mar;17(3):705-21. (PMID: 15722475)
Hortic Res. 2023 Jul 05;10(8):uhad135. (PMID: 37694228)
Methods Mol Biol. 2007;406:179-212. (PMID: 18287693)
Nucleic Acids Res. 2021 Jan 8;49(D1):D412-D419. (PMID: 33125078)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
Int J Mol Sci. 2015 Jul 13;16(7):15811-51. (PMID: 26184177)
Nucleic Acids Res. 1999 Jan 1;27(1):295-6. (PMID: 9847207)
J Mol Evol. 1998 Jan;46(1):74-83. (PMID: 9419227)
Plant Cell Physiol. 2016 Aug;57(8):1657-77. (PMID: 27279646)
Plant Sci. 2021 Jul;308:110924. (PMID: 34034872)
PLoS One. 2010 Jun 28;5(6):e11335. (PMID: 20596258)
Plant J. 2015 Jun;82(6):962-977. (PMID: 25912720)
Int J Mol Sci. 2021 Jun 07;22(11):. (PMID: 34200125)
Plant J. 2007 Nov;52(3):528-38. (PMID: 17727613)
BMC Genomics. 2012 Oct 10;13:544. (PMID: 23050870)
Nucleic Acids Res. 2009 Jul;37(Web Server issue):W202-8. (PMID: 19458158)
Int J Mol Sci. 2020 Nov 04;21(21):. (PMID: 33158201)
EMBO J. 1987 Dec 1;6(12):3553-8. (PMID: 3428265)

PL202403 Open funds of the Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding; BE2022336 and JBGS[2021]001 Jiangsu Province Government; BM2022008-02 Project of Zhongshan Biological Breeding Laboratory; B21HJ0220 Hainan Yazhou Bay Seed Lab; 22KJA210003 Natural Science Foundation of the Jiangsu Higher Education Institutions of China

Keywords: Gene expression; R2R3-MYB; Rice (Oryza sativa L.); Salt stress; Transcription factor

0 (Transcription Factors)
0 (Plant Proteins)

Date Created: 20240823 Date Completed: 20240824 Latest Revision: 20240826

20240826

PMC11342600

10.1186/s12864-024-10693-5

39179980