Single-cell transcriptomic atlas of taste papilla aging.

Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101130839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1474-9726 (Electronic) Linking ISSN: 14749718 NLM ISO Abbreviation: Aging Cell Subsets: MEDLINE

Publication: Oxford, UK : Wiley-Blackwell
Original Publication: Oxford, UK : Blackwell Pub., c2002-

Aging*/genetics ; Transcriptome*/genetics ; Taste Buds*/metabolism ; Single-Cell Analysis*; Animals ; Mice

Taste perception is one of the important senses in mammals. Taste dysfunction causes significant inconvenience in daily life, leading to subhealth and even life-threatening condition. Aging is a major cause to taste dysfunction, while the underlying feature related to gustatory aging is still not known. Using single-cell RNA Sequencing, differentially expressed genes between aged and young taste papillae are identified, including upregulated mt-Nd4l and Xist, as well as downregulated Hsp90ab1 and Tmem59. In the Tmem59 ^-/- circumvallate papillae (CVP), taste mature cell generation is impaired by reduction in the numbers of PLCβ2 ⁺ and Car4 ⁺ cells, as well as decreases in expression levels of taste transduction genes. Tmem59 ^-/- mice showed deficits in sensitivities to tastants. Through screening by GenAge and DisGeNET databases, aging-dependent genes and oral disease-associated genes are identified in taste papillae. In the CVP, aging promotes intercellular communication reciprocally between (cycling) basal cell and mature taste cell by upregulated Crlf1/Lifr and Adam15/Itga5 signaling. By transcriptional network analysis, ribosome proteins, Anxa1, Prdx5, and Hmgb1/2 are identified as transcriptional hubs in the aged taste papillae. Chronological aging-associated transcriptional changes throughout taste cell maturation are revealed. Aged taste papillae contain more Muc5b ⁺ cells that are not localized in gustatory gland. Collectively, this study shows molecular and cellular features associated with taste papilla aging.
(© 2024 The Author(s). Aging Cell published by Anatomical Society and John Wiley & Sons Ltd.)

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GWVI-11.2-XD09 Shanghai Municipal Health Commission; GZ2022006 Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning; 31900714 National Natural Science Foundation of China; 32070996 National Natural Science Foundation of China; 32271044 National Natural Science Foundation of China; 82271136 National Natural Science Foundation of China; 21140900600 Science and Technology Innovation Plan Of Shanghai Science and Technology Commission; 23ZR1409600 Natural Science Foundation of Shanghai Municipality

Keywords: aging; circumvallate papillae; foliate papillae; mature taste cell; single‐cell RNA sequencing

Date Created: 20240821 Date Completed: 20241212 Latest Revision: 20241214

20241214

PMC11634696

10.1111/acel.14308

39169434