The influence of rutin and chlorogenic acid on oxidative stress and in vivo fertility: Evaluation of the quality and antioxidant status of post-thaw semen from Azari water buffalo bulls.

Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 101678837 Publication Model: Print Cited Medium: Internet ISSN: 2053-1095 (Electronic) Linking ISSN: 20531095 NLM ISO Abbreviation: Vet Med Sci Subsets: MEDLINE

Original Publication: [Oxford] : John Wiley & Sons Ltd., [2015]-

Buffaloes*/physiology ; Rutin*/pharmacology ; Oxidative Stress*/drug effects ; Chlorogenic Acid*/pharmacology ; Semen Preservation*/veterinary ; Semen Preservation*/methods ; Antioxidants*/pharmacology ; Semen Analysis*/veterinary ; Fertility*/drug effects ; Cryopreservation*/veterinary; Animals ; Male ; Semen/drug effects ; Semen/physiology ; Female ; Spermatozoa/drug effects ; Spermatozoa/physiology ; Dose-Response Relationship, Drug

Background: The vulnerability of buffalo sperm to cryoinjury necessitates the improvement of sperm cryo-resistance as a critical strategy for the widespread use of assisted reproductive technologies in buffalo.
Objectives: The main aim of the present study was to evaluate the effects of different concentrations of rutin and chlorogenic acid (CGA) on buffalo semen quality, antioxidant activity and fertility during cryopreservation.
Methods: The semen was collected and pooled from the 3 buffaloes using an artificial vagina (18 ejaculations). The pooled sperm were divided into nine different groups: control (Tris-based extender); 0.4, 0.6, 0.8 and 1 mM rutin (rutin + Tris-based extender); and 50, 100, 150 and 200 µM CGG (CGA + Tris-based extender). Sperm kinematics, viability, hypo-osmotic swelling test, mitochondrial activity, antioxidant activities and malondialdehyde (MDA) concentration of frozen and thawed buffalo sperm were evaluated. In addition, 48 buffalo were finally inseminated, and pregnancy was rectally determined 1 month after insemination.
Results: Compared to the control group, adding R-0.4, R-0.6, CGA-100 and CGA-150 can improve total and progressive motility, motility characteristics, viability, PMF and DNA damage in buffalo sperm. In addition, the results showed that R-0.4, R-0.6, CGA-50, CGA-100 and CGA-150 increased total antioxidant capacity, catalase, glutathione peroxidase and glutathione activities and decreased MDA levels compared to the control group. Furthermore, it has been shown that adding 150 µM CGA and 0.6 mM rutin to an extender can increase in vivo fertility compared to the control group.
Conclusions: In conclusion, adding rutin and CGA to the extender improves membrane stability and in vivo fertility of buffalo sperm by reducing oxidative stress.
(© 2024 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd.)

J Dairy Sci. 2013 Jan;96(1):300-6. (PMID: 23141825)
Theriogenology. 2009 May;71(8):1326-9. (PMID: 19246080)
Reprod Domest Anim. 2020 Sep;55(9):1229-1239. (PMID: 32644236)
Cryobiology. 2024 Jun;115:104868. (PMID: 38423495)
Tissue Cell. 2018 Feb;50:133-143. (PMID: 29429513)
Theriogenology. 2020 Mar 15;145:190-197. (PMID: 31732163)
Avicenna J Phytomed. 2020 Jan-Feb;10(1):35-49. (PMID: 31921606)
Vet Res Forum. 2013 Summer;4(3):185-9. (PMID: 25653795)
Vet Res Forum. 2012 Fall;3(4):263-8. (PMID: 25653769)
Theriogenology. 2022 Feb;179:32-38. (PMID: 34823059)
Avicenna J Phytomed. 2018 Sep-Oct;8(5):448-456. (PMID: 30345232)
AAPS PharmSciTech. 2018 May;19(4):1773-1780. (PMID: 29600391)
Theriogenology. 2019 Aug;134:18-23. (PMID: 31112913)
Food Chem. 2018 Apr 25;246:48-57. (PMID: 29291876)
Theriogenology. 2012 Sep 15;78(5):1005-19. (PMID: 22818091)
J Anim Sci. 2010 May;88(5):1657-62. (PMID: 20081087)
Andrologia. 2016 Nov;48(9):855-861. (PMID: 26762772)
J Agric Food Chem. 2007 Mar 7;55(5):2020-7. (PMID: 17286412)
Reprod Domest Anim. 2009 Apr;44(2):345-9. (PMID: 19144010)
Anim Reprod Sci. 2009 Aug;114(1-3):125-34. (PMID: 19010614)
Theriogenology. 2016 Oct 1;86(6):1516-1522. (PMID: 27321805)
Biol Reprod. 2013 Mar 21;88(3):68. (PMID: 23325813)
Front Vet Sci. 2023 Apr 27;10:1119153. (PMID: 37180054)
Anim Sci J. 2020 Jan;91(1):e13328. (PMID: 32219925)
Syst Biol Reprod Med. 2023 Oct;69(5):366-378. (PMID: 37225677)
Anim Reprod Sci. 2016 Apr;167:83-8. (PMID: 26896924)
Theriogenology. 2007 Mar 15;67(5):1060-7. (PMID: 17280711)
Cryobiology. 2021 Aug;101:12-19. (PMID: 34245722)
Cryobiology. 2023 Sep;112:104557. (PMID: 37451667)
Andrologia. 2014 Sep;46(7):777-83. (PMID: 23957377)
Sci Rep. 2023 Jul 5;13(1):10873. (PMID: 37407620)
Clin Chem Lab Med. 2008;46(3):342-9. (PMID: 18254708)
Vet Med Sci. 2023 Jul;9(4):1791-1802. (PMID: 37197763)
Anim Reprod Sci. 2010 Jun;119(3-4):183-90. (PMID: 20197223)
Theriogenology. 2006 Sep 15;66(5):1115-22. (PMID: 16620943)
J Cell Physiol. 2018 Jul;233(7):5267-5280. (PMID: 29231961)
Biol Reprod. 2002 Feb;66(2):354-60. (PMID: 11804948)
Anim Reprod Sci. 2006 Jul;93(3-4):246-57. (PMID: 16198514)
Fertil Steril. 2018 Nov;110(6):1058-1066. (PMID: 30396550)
Cell Biochem Biophys. 2019 Mar;77(1):99-107. (PMID: 30218405)
Anim Reprod. 2020 Jan 24;17(1):e20190021. (PMID: 32368276)
Cryobiology. 2023 Jun;111:104-112. (PMID: 37142111)
Theriogenology. 2017 Jan 15;88:1-8. (PMID: 27865407)
Int J Fertil Steril. 2016 Jul-Sep;10(2):215-23. (PMID: 27441055)
Mutat Res. 2002 Jan 15;513(1-2):193-6. (PMID: 11719104)
Theriogenology. 2023 Feb;197:94-100. (PMID: 36476507)
Reprod Domest Anim. 2014 Feb;49(1):122-5. (PMID: 24112366)
Evid Based Complement Alternat Med. 2013;2013:801457. (PMID: 24062792)
Andrologia. 2017 Feb;49(1):. (PMID: 27106863)
Theriogenology. 2012 May;77(8):1497-504. (PMID: 22225691)
Life Sci. 2015 Apr 15;127:12-8. (PMID: 25744394)
Reprod Biol. 2012 Nov;12(3):271-6. (PMID: 23153697)
Mol Reprod Dev. 1995 Nov;42(3):334-46. (PMID: 8579848)
Mol Reprod Dev. 2001 Dec;60(4):498-506. (PMID: 11746961)
Cryobiology. 2020 Aug;95:29-35. (PMID: 32590017)
Anim Reprod Sci. 2017 Apr;179:20-26. (PMID: 28189350)
Reprod Domest Anim. 2018 Dec;53(6):1600-1604. (PMID: 30053311)
Eur Rev Med Pharmacol Sci. 2013 Feb;17(4):491-500. (PMID: 23467948)

Keywords: chlorogenic acid; rutin; sperm cryo‐resistance; thawed buffalo sperm

5G06TVY3R7 (Rutin)
318ADP12RI (Chlorogenic Acid)
0 (Antioxidants)

Date Created: 20240819 Date Completed: 20240819 Latest Revision: 20240821

20250114

PMC11332393

10.1002/vms3.1548

39158970