Strigolactones Negatively Regulate Tobacco Mosaic Virus Resistance in Nicotiana benthamiana .

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Nicotiana*/virology ; Nicotiana*/genetics ; Nicotiana*/immunology ; Tobacco Mosaic Virus*/physiology ; Lactones*/pharmacology ; Disease Resistance*/genetics ; Gene Expression Regulation, Plant* ; Plant Diseases*/virology ; Plant Diseases*/genetics ; Plant Diseases*/immunology; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plant Growth Regulators/metabolism ; Plant Growth Regulators/pharmacology ; Plant Immunity/genetics ; Plant Immunity/drug effects ; Gene Silencing

Strigolactones (SLs) are plant hormones that regulate diverse developmental processes and environmental responses in plants. It has been discovered that SLs play an important role in regulating plant immune resistance to pathogens but there are currently no reports on their role in the interaction between Nicotiana benthamiana and the tobacco mosaic virus (TMV). In this study, the exogenous application of SLs weakened the resistance of N. benthamiana to TMV, promoting TMV infection, whereas the exogenous application of Tis108, a SL inhibitor, resulted in the opposite effect. Virus-induced gene silencing (VIGS) inhibition of two key SL synthesis enzyme genes, NtCCD7 and NtCCD8 , enhanced the resistance of N. benthamiana to TMV. Additionally, we conducted a screening of N. benthamiana related to TMV infection. TMV-infected plants treated with SLs were compared to the control by using RNA-seq. The KEGG enrichment analysis and weighted gene co-expression network analysis (WGCNA) of differentially expressed genes (DEGs) suggested that plant hormone signaling transduction may play a significant role in the SL-TMV- N. benthamiana interactions. This study reveals new functions of SLs in regulating plant immunity and provides a reference for controlling TMV diseases in production.

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32302373 National Natural Science Foundation of China; kq2208139 Changsha Municipal Natural Science Foundation; 2023JJ40394 Hunan Provincial Natural Science Foundation; CARS-16-E17 China Agriculture Research System; 2022CX107 Hunan Provincial Agricultural Science and Technology Innovation Fund; 32302300 National Natural Science Foundation of China; 2021-620-003-001 Hubei Provincial Agricultural Science and Technology Innovation Center Project; 2023CX40 Hunan Provincial Agricultural Science and Technology Innovation Fund

Keywords: RNA sequencing; plant hormones; plant immunity; strigolactones; tobacco mosaic virus

0 (Lactones)
0 (Plant Proteins)
0 (Plant Growth Regulators)

Date Created: 20240810 Date Completed: 20240810 Latest Revision: 20240812

20240813

PMC11313310

10.3390/ijms25158518

39126086